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Pseudomonas humi sp. nov., isolated from leaf soil

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Abstract

An aerobic, Gram-negative, non-sporulating, motile, rod-shaped and lignin-degrading bacterial strain, Pseudomonas sp. CCA1, was isolated from leaf soil collected in Japan. This strain grew at 20–45 °C (optimum 20 °C), at pH 5.0–10.0 (optimum pH 5.0), and in the presence of 2% NaCl. Its major cellular fatty acids were C16:0 and summed feature 8 (C18:1ω6c and/or C18:1ω7c). The predominant quinone system was ubiquinone-9. Comparative 16S rRNA gene sequence analysis showed that Pseudomonas sp. CCA1 was related most closely to P. citronellolis NBRC 103043T (98.9%), but multilocus sequence analysis based on fragments of the atpD, gyrA, gyrB and rpoB gene sequences showed strain CCA1 to branch separately from its most closely related Pseudomonas type strains. DNA–DNA hybridization values between Pseudomonas sp. CCA1 and type strains of closely related Pseudomonas species were less than 53%. Based on its phenotypic, chemotaxonomic and phylogenetic features, we propose that Pseudomonas sp. CCA1 represents a novel species within the genus Pseudomonas, for which the name Pseudomonas humi sp. nov. is proposed. The type strain is CCA1 (= HUT 8136T = TBRC 8616T).

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Funding

This work was supported in part by the Science and Technology Research Partnership for Sustainable Development (SATREPS), under the Japan Science and Technology Agency (JST) and Japan International Cooperation Agency (JICA).

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Authors and Affiliations

  1. Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-0046, Japan

    Hironaga Akita & Tamotsu Hoshino

  2. Department of Civil and Environmental Engineering, National Institute of Technology, Kure College, 2-2-11 Aga-minami, Kure, Hiroshima, 737-8506, Japan

    Zen-ichiro Kimura

  3. Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, Sapporo, 062-8517, Japan

    Tamotsu Hoshino

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  1. Hironaga Akita
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  2. Zen-ichiro Kimura
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  3. Tamotsu Hoshino
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Correspondence to Zen-ichiro Kimura.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Communicated by Erko Stackebrandt.

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Akita, H., Kimura, Zi. & Hoshino, T. Pseudomonas humi sp. nov., isolated from leaf soil. Arch Microbiol 201, 245–251 (2019). https://doi.org/10.1007/s00203-018-1588-x

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  • DOI: https://doi.org/10.1007/s00203-018-1588-x

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