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Lysobacter tongrenensis sp. nov., isolated from soil of a manganese factory

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Abstract

A Gram-staining negative, aerobic, non-motile, rod-shaped bacterial strain, designated YS-37T, was isolated from soil in a manganese factory, People’s Republic of China. Based on16S rRNA gene sequence analysis, strain YS-37T was most closely related to Lysobacter pocheonensis Gsoil 193T (97.0%), Lysobacter dokdonensis DS-58T (96.0%) and Lysobacter daecheongensis Dae08T (95.8%) and grouped together with L. pocheonensis Gsoil 193T and Lysobacter dokdonensis DS-58T. The DNA–DNA hybridization value between strain YS-37T and L. pocheonensis KCTC 12624T was 43.3% (± 1). The major respiratory quinone of strain YS-37T was ubiquinone-8, and the polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phospholipid, phosphatidylmethylethaolamine and two unknown lipids. Its major cellular fatty acids (> 5%) were iso-C15:0, iso-C17:1 ω9c, iso-C16:0, iso-C11:0 3-OH and iso-C11:0 and the G + C content of the genomic DNA was 67.1 mol%. Strain YS-37T also showed some biophysical and biochemical differences with the related strains, especially in hydrolysis of casein. The results demonstrated that strain YS-37T belongs to genus Lysobacter and represents a novel Lysobacter species for which the name Lysobacter tongrenensis sp. nov. is proposed. The type strain is YS-37T (= CCTCC AB 2016052T = KCTC 52206T).

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Acknowledgements

We are grateful to Dr. Bernhard Schink for the etymology of the strain’s name and Penghao Cai for technique assistance. The analyses of quinone, polar lipid and DNA G + C content were performed by DSMZ. This work was supported by National Key Research and Development Program of China (2016YFD0800702) and China Postdoctoral Science Foundation Special Funding (2015T80814).

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Author notes

  1. Jingxin Li and Yushan Han contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, People’s Republic of China

    Jingxin Li, Yushan Han, Wei Guo, Qian Wang, Shuijiao Liao & Gejiao Wang

  2. College of Basis Science, Huazhong Agricultural University, Wuhan, Hubei, 430070, People’s Republic of China

    Shuijiao Liao

  3. Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, MT, 59717, USA

    Qian Wang

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  1. Jingxin Li
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  2. Yushan Han
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  3. Wei Guo
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  4. Qian Wang
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Correspondence to Gejiao Wang.

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Communicated by Erko Stackebrandt.

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Li, J., Han, Y., Guo, W. et al. Lysobacter tongrenensis sp. nov., isolated from soil of a manganese factory. Arch Microbiol 200, 439–444 (2018). https://doi.org/10.1007/s00203-017-1457-z

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  • DOI: https://doi.org/10.1007/s00203-017-1457-z

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