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Investigating the compatibility of the biocontrol agent Clonostachys rosea IK726 with prodigiosin-producing Serratia rubidaea S55 and phenazine-producing Pseudomonas chlororaphis ToZa7

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Abstract

This study was carried out to assess the compatibility of the biocontrol fungus Clonostachys rosea IK726 with the phenazine-producing Pseudomonas chlororaphis ToZa7 or with the prodigiosin-producing Serratia rubidaea S55 against Fusarium oxysporum f. sp. radicis-lycopersici. The pathogen was inhibited by both strains in vitro, whereas C. rosea displayed high tolerance to S. rubidaea but not to P. chlororaphis. We hypothesized that this could be attributed to the ATP-binding cassette (ABC) proteins. The results of the reverse transcription quantitative PCR showed an induction of seven genes (abcB1, abcB20, abcB26, abcC12, abcC12, abcG8 and abcG25) from subfamilies B, C and G. In planta experiments showed a significant reduction in foot and root rot on tomato plants inoculated with C. rosea and P. chlororaphis. This study demonstrates the potential for combining different biocontrol agents and suggests an involvement of ABC transporters in secondary metabolite tolerance in C. rosea.

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Acknowledgments

This scientific work was co-funded by the project “Grants of the State Scholarships Foundation of Greece”, using funds of the European Project “Education and Lifelong Learning”, from the European Social Fund (ESF) of the National Strategic Reference Framework (NSRF) 2007–2013. The work was also financially supported by the Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences and the Swedish Research Council for Agricultural Sciences and Spatial Planning, Formas (Grant No. 229-2012-1288). We thank Prof. Ben Lugtenberg, Institute of Biology, Leiden University, The Netherlands, for kindly providing Forl (isolate ZUM 2407/IPO-DLO).

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  1. Georgios Tzelepis

    Present address: Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, Box 7080, 75007, Uppsala, Sweden

Authors and Affiliations

  1. Laboratory of Plant Pathology, School of Agriculture, Aristotle University of Thessaloniki, P.O. Box 269, 541 24, Thessaloniki, Greece

    Nathalie N. Kamou & Anastasia L. Lagopodi

  2. Department of Forest Mycology and Plant Pathology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Box 7026, 75007, Uppsala, Sweden

    Mukesh Dubey, Georgios Tzelepis, Magnus Karlsson & Dan Funck Jensen

  3. Laboratory of Agronomy, School of Agriculture, Aristotle University of Thessaloniki, P.O. Box 269, 541 24, Thessaloniki, Greece

    Georgios Menexes

  4. Laboratory of Pesticide Science, Department of Agriculture, Aristotle University of Thessaloniki, P.O. Box 1678, 540 06, Thessaloniki, Greece

    Emmanouil N. Papadakis

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  1. Nathalie N. Kamou
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Correspondence to Nathalie N. Kamou.

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Communicated by Jorge Membrillo-Hernández.

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Kamou, N.N., Dubey, M., Tzelepis, G. et al. Investigating the compatibility of the biocontrol agent Clonostachys rosea IK726 with prodigiosin-producing Serratia rubidaea S55 and phenazine-producing Pseudomonas chlororaphis ToZa7. Arch Microbiol 198, 369–377 (2016). https://doi.org/10.1007/s00203-016-1198-4

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