Abstract
This study aimed at increasing carotenoid yield of Rhodobacter sphaeroides in wastewater treatment by adding magnesium ion (Mg2+). Results showed that Mg2+ could improve R. sphaeroides biomass and carotenoid yield effectively. The highest carotenoid yield of 4.83 ± 0.14 mg/g biomass and biomass production of 3900 ± 180 mg/L were achieved at optimal Mg2+ concentration of 15 mmol/L. Mechanism analysis revealed that Mg2+ could promote carotenoid production by regulating the expressions of crt genes. Up-regulation of crtBDA genes improved carotenoid biosynthesis of R. sphaeroides.
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This study was financially supported by the National Natural Science Foundation of China (51278489).
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Communicated by Reinhard Krämer.
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Liu, S., Li, X., Zhang, G. et al. Effect of magnesium ion on crt gene expression in improving carotenoid yield of Rhodobacter sphaeroides . Arch Microbiol 197, 1101–1108 (2015). https://doi.org/10.1007/s00203-015-1150-z
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DOI: https://doi.org/10.1007/s00203-015-1150-z