Abstract
The photosynthetic bacterium, Rhodobacter sphaeroides, is a bacterium that can grow in a variety of environments and produces substances with antioxidant effects. In this study, we investigated the culture conditions to increase the production of antioxidants in R. sphaeroides, which can grow under both aerobic and anaerobic conditions. After incubation in the exponential phase and stationary phase under both conditions, a 2,2-diphenyl-1-picrylhydrazyl assay was used to confirm the antioxidant effect. Although the highest antioxidant effect was shown in the stationary phase under aerobic conditions, the antioxidant effect of each cell was found to be greater when cultured under anaerobic conditions. The antioxidant activity of R. sphaeroides was increased by sonication. In addition, the contents of carotenoids and bacteriochlorophyll, which are pigments with antioxidant effects, produced by R. sphaeroides were measured. We confirmed that the content of carotenoids was higher in anaerobic conditions than in aerobic conditions. However, when measuring the content of the bacterium, we found that there was more content in aerobic conditions. Therefore, we confirm that when grown in anaerobic conditions, the antioxidant effect of R. sphaeroides is higher, which suggests that this antioxidant effect comes from the effect of carotenoid.
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Acknowledgements
This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, and Forestry (IPET) through the Crop Viruses and Pests Response Industry Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (321108-04).
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SL performed all of the experiments. SL and JY wrote the main manuscript text. Y-HK and JM designed the overall experimental concept and revised the manuscript; approved the final version: all authors.
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Subin Lee, Jaeyoung Yu, Yang-Hoon Kim, and Jiho Min declare that they have no conflict of interest.
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Lee, S., Yu, J., Kim, YH. et al. Optimized Rhodobacter sphaeroides for the Production of Antioxidants and the Pigments with Antioxidant Activity. Mol Biotechnol 65, 131–135 (2023). https://doi.org/10.1007/s12033-022-00547-4
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DOI: https://doi.org/10.1007/s12033-022-00547-4