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Sphingomonas panaciterrae sp. nov., a plant growth-promoting bacterium isolated from soil of a ginseng field

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Abstract

Strain DCY91T, a Gram-stain-negative, rod-shaped, aerobic, non-motile bacterium, was isolated from soil of ginseng field in Gyeonggi province, South Korea. Strain DCY91T shared the highest 16S rRNA gene sequence similarity with Sphingomonas mucosissima DSM 17494T (98.55 %), Sphingomonas dokdonensis KACC 17420T (98.11 %) and Sphingomonas xinjiangensis DSM 26736T (96.68 %). The strain DCY91T was found to able to grow best in trypticase soy agar at 28 °C, at pH 7 and at 0.5 % NaCl. Ubiquinone 10 was identified as the isoprenoid quinone. The major polar lipids were identified as sphingoglycolipid, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The major fatty acids of strain DCY91T were identified as unsaturated C18:1 ω7c and saturated C16:0. The major polyamine content was sym-homospermidine. The DNA G + C content was determined to be 65.8 mol% (HPLC). After 6 days of incubation, strain DCY91T produced 9.64 ± 1.73 and 33.73 ± 4.66 µg/ml indole-3-acetic acid, using media without l-tryptophan and supplemented with l-tryptophan, respectively. Strain DCY91T was also weakly solubilized phosphate and produced siderophores. On the basis of the phenotypic characteristics, genotypic analysis and chemotaxonomic characteristics, strain DCY91T is considered to represent a novel species of the genus Sphingomonas, for which the name Sphingomonas panaciterrae sp. nov. is proposed. The type strain is DCY91T (=KCTC 42346T =JCM 30807T).

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Acknowledgments

This research was supported by Institute of Planning and Evaluation for Technology (IPET #313038-03-1-S13020) in Food, Agriculture, Forestry and Fisheries, Republic of Korea, and also supported by a grant from the Next-Generation BioGreen 21 Program, Systems and Synthetic Agrobiotech Center (SSAC # PJ01116602), Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Graduate School of Biotechnology and Ginseng Bank, College of Life Science, Kyung Hee University, Yongin, 446-701, Korea

    Johan Sukweenadhi, Mohamed El-Agamy Farh & Deok-Chun Yang

  2. Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin, 446-701, Korea

    Yeon-Ju Kim, Ngoc-Lan Nguyen, Van-An Hoang & Deok-Chun Yang

  3. Division of Applied Life Science and PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju, 660-701, Korea

    Chang Ho Kang

  4. GyeonggiDo Agricultural Research and Extension Services, Hwaseong, Gyeonggi-do, Korea

    Eul-Su Choi

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  1. Johan Sukweenadhi
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  4. Mohamed El-Agamy Farh
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Correspondence to Yeon-Ju Kim or Deok-Chun Yang.

Additional information

Communicated by Erko Stackebrandt.

Electronic supplementary material

Below is the link to the electronic supplementary material.

203_2015_1134_MOESM1_ESM.tif

Supplementary material 1 The maximum-parsimony tree based on 16S rRNA gene sequence analysis showing phylogenetic relationships of strain DCY91T and members of the genus Sphingomonas (TIFF 10997 kb)

203_2015_1134_MOESM2_ESM.tif

Supplementary material 2 Two-dimensional TLC of the total polar lipids of Sphingomonas panaciterrae DCY91T (a) and S. mucosissima DSM 17494T (b), stained for total polar lipids with 5 % ethanolic molybdophosphoric acid. Abbreviations: SGL, sphingoglycolipid; DPG, diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PC, phosphatidylcholine; L, unidentified lipid (TIFF 15383 kb)

Supplementary material 3 (DOCX 14 kb)

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Sukweenadhi, J., Kim, YJ., Kang, C.H. et al. Sphingomonas panaciterrae sp. nov., a plant growth-promoting bacterium isolated from soil of a ginseng field. Arch Microbiol 197, 973–981 (2015). https://doi.org/10.1007/s00203-015-1134-z

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  • DOI: https://doi.org/10.1007/s00203-015-1134-z

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