Summary
Genetic analyses of n-alkane non-utilizing mutants of the yeast Yarrowia (Saccharomycopsis) lipolytica were continued. By analyses of inter-mutant complementation and recombination a total of 26 genetic loci have been identified. Mutations representing these loci have phenotypes characteristic of defects in substrate uptake or in one or more of the enzymatic activities making up the hydroxylase complex. Tests of 14C n-hexadecane uptake by a set of alkane-negative mutants representing the 26 loci show that 16 of the mutations cause a significant reduction in n-alkane uptake. N-alkane uptake by Y. lipolytica is shown to be inducible and to be inhibited by the metabolic poisons 2–4 dinitrophenol and KCN. The latter observation indicates that n-alkane uptake of Y. lipolytica is due to active transport.
Similar content being viewed by others
References
Armitt S, McCullough W, Roberts CF (1976) J Gen Microbiol 92:263–282
Bassel JB, Mortimer RK (1982) Curr Genet 5:77–88
Douglas HC, Hawthorne DC (1972) J Bacteriol 109:1139–1143
Gilewicz M, Zacek M, Bertrand JC, Azoulay E (1978) Can J Microbiol 25:201–206
Goma G, Parielleux A, Durand G (1973) J Ferment Technol 51:616–618
Hug H, Blanch HW, Fiechter A (1974) Biotechnol Bioeng 16:965–985
Kappelli O, Fiechter A (1980) Biotechnol. Bioeng 22:1829–1841
Kappelli O, Muller M, Fiechter A (1978) J Bacteriol 133:952–958
Kawamoto S, Nozaki C, Tanaka A, Fukui S (1978) Eur J Biochem 83:609–613
Lebeault JM, Roche B, Duvnjak Z, Azoulay E (1970) Biochem Biophys Acta 220:373–379
Lehninger AL (1975) Biochemistry, 2nd edn. Worth, p 495, p 519
Liu CM, Johnson M (1971) J Bacteriol 106:830–834
Miura Y, Okazaki M, Hamada SI, Masakawa SI, Yugen (1977) Biotechnol Bioeng 19:701–714
Munk V, Dostalek M, Volfova O (1969) Biotechnol Bioeng 11:383–391
Ogrydziak D, Bassel J, Contopoulou R, Mortimer R (1978) Mol Gen Genet 163:229–239
Ogrydziak D, Bassel J, Mortimer R (1978) Mol Gen Genet 188:179–183
Ogrydziak DM, Mortimer RK (1977) Genetics 87:621–632
Osumi M, Miwa N, Tanaka Y, Fukui S (1974) Arch Microbiol 99:181–184
Reddy P, Singh H, Roy P, Baruah J (1982) Biotechnol Bioeng 24:1241–1269
Yanagashima N (1978) Bot Mag Tokyo Special Issue 1:61–81
Yorifugi T (1978) n-alkane oxidation by Saccharomycopsis lipolytica. PhD Thesis, Massachusetts Institute of Technology
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bassel, J.B., Mortimer, R.K. Identification of mutations preventing n-hexadecane uptake among 26 n-alkane non-utilizing mutants of Yarrowia (Saccharomycopsis) lipolytica . Curr Genet 9, 579–586 (1985). https://doi.org/10.1007/BF00381171
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00381171