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Production, purification and structural characterization of an exopolysaccharide produced by a probiotic Lactobacillus plantarum MTCC 9510

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Abstract

Exopolysaccharides (EPS) from lactic acid bacteria contribute to specific rheology and texture of fermented milk products and finds applications even in non-dairy foods and in therapeutics. Box-Behnken model of response surface methodology (RSM) was employed to formulate the production medium for exopolysaccharide (EPS). FT-IR spectral analysis of the purified EPS from Lactobacillus plantarum MTCC 9510 revealed prominent characteristic groups corresponding to polyhydric alcohols. The degradation temperature (Td) of the polysaccharide was found to be 260°C with the help of thermo gravimetric analysis (TGA). Structure elucidation of the EPS showed that it consists of a trisaccharide repeating unit of α-d-glucose, β-d-glucose and α-d-mannose.

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Acknowledgments

The SRF fellowship from the Council of Scientific and Industrial Research (CSIR), New Delhi to BI is greatly acknowledged. The Programme was supported by DBT, New Delhi. The authors would like to acknowledge Mr. Sankar Sasidharan, Materials and Minerals Division, NIIST, Trivandrum for technical support.

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Authors and Affiliations

  1. Biotechnology Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Trivandrum, 695 019, Kerala, India

    Bindhumol Ismail & Kesavan Madhavan Nampoothiri

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  1. Bindhumol Ismail
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  2. Kesavan Madhavan Nampoothiri
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Correspondence to Kesavan Madhavan Nampoothiri.

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Communicated by Ercko Stackebrandt.

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Ismail, B., Nampoothiri, K.M. Production, purification and structural characterization of an exopolysaccharide produced by a probiotic Lactobacillus plantarum MTCC 9510. Arch Microbiol 192, 1049–1057 (2010). https://doi.org/10.1007/s00203-010-0636-y

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  • DOI: https://doi.org/10.1007/s00203-010-0636-y

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