Skip to main content
SpringerLink
Log in

Evaluation of the Probiotic Characteristics of Newly Isolated Lactic Acid Bacteria

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Lactic acid bacteria were isolated from fermented vegetables, sour dough, milk products, sheep and human excreta. The newly isolated cultures were evaluated for a number of probiotic characteristics like bile salt resistance, salt tolerance in general, survival in low pH, hydrophobicity of the cell surface, resistance to low phenol concentration, antimicrobial activity and susceptibility pattern against vancomycin and erythromycin. The selected cultures were further screened for their ability to produce the nutraceticals such as folic acid and exopolysaccharide (EPS). Two potent isolates, CB2 (from cabbage) and SD2 (from sour dough) were found to produce both extracellular and intracellular folate. One of the isolates from yogurt (MC-1) and the one from whey (W3) produced significant amount of EPS with a maximum production of 8.79 ± 0.05 g/l by MC-1.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Bolin, Z., Libudzisz, Z., & Moneta, J. (1997). Polish Journal of Food and Nutrition Sciences, 6, 71–78.

    CAS  Google Scholar 

  2. Fuller, R. (1989). Journal of Applied Bacteriology, 66, 365–378.

    CAS  Google Scholar 

  3. Holzapfel, W. H., Haberer, P., Geisen, R., Bjorkroth, J., & Schillinger, U. (2001). American Journal of Clinical Nutrition, 73, 365S–373S.

    CAS  Google Scholar 

  4. Molly, K., De Smet, I., Nollet, L., Van de Woestyne, M., & Ver-straete, W. (1996). Microbial Ecology in Health and Disease, 9, 79–89.

    Article  Google Scholar 

  5. Salminen, S., Laine, M., von Wright, A., et al. (1996). Bioscience and Microflora, 15, 61–67.

    Google Scholar 

  6. Brower, V. (1998). Nature Biotechnology, 16, 728–731.

    Article  CAS  Google Scholar 

  7. Temmerman, R., Huys, G., & Swings, J. (2004). Trends in Food Science & Technology, 15, 348–359.

    Article  CAS  Google Scholar 

  8. Crescenzi, V. (1995). Biotechnology Progress, 11, 251–259.

    Article  CAS  Google Scholar 

  9. Giraud, E., Brauman, A., Keleke, S., Leong, B., & Raimbault, M. (1991). Applied Microbiology and Biotechnology, 36, 379–383.

    CAS  Google Scholar 

  10. Sharpe, M. E. (1979). In F. A. Skinner, & D. W. Lovelock (Eds.) Identification methods for microbiologists pp. 233–259. London: Academic.

  11. Barker, S. B., & Summerson, W. H. (1941). Journal of Biological Chemistry, 138, 535–554.

    CAS  Google Scholar 

  12. Miller, G. L. (1959). Analytical Chemistry, 31, 426–429.

    Article  CAS  Google Scholar 

  13. Thapa, N., Pal, J., & Tamang, J. P. (2004). World Journal of Microbiology & Biotechnology, 20, 599–607.

    Article  CAS  Google Scholar 

  14. Savadogo, A., Outtara, C., Savadogo, P., et al. (2004). African Journal of Biotechnology, 3, 189–194.

    CAS  Google Scholar 

  15. Dubois, M., Gilles, K., Hamilton, J., Rebers, P., & Smith, F. (1956). Analytical Chemistry, 28, 350–356.

    Article  CAS  Google Scholar 

  16. Holt, D. L., Wehling, R. L., & Zeece, M. G. (1988). Journal of Chromatography, 449, 271–279.

    Article  CAS  Google Scholar 

  17. Lin, M. Y., & Young, C. M. (2000). International Dairy Journal, 10, 409–414.

    Article  CAS  Google Scholar 

  18. Holt, J. H., Krieg, N. R., Sneath, P. H. A., Staley, J. T., & Williams, S. T. (1994). In W. R. Hensyl (Ed.) Bergey’s Manual of Determinative Bacteriology (9th ed.). Baltimore, MD, USA: Williams & Wilkins.

  19. Erkkilä, S., & Petäjä, E. (2000). Meat Science, 55, 297–300.

    Article  Google Scholar 

  20. Adnan, A. F. M., & Tan, I. K. P. (2007). Bioresource Technology, 98, 1380–1385.

    Article  CAS  Google Scholar 

  21. Gilliland, S. E., & Walker, D. K. (1990). Journal of Dairy Science, 73, 905–911.

    Article  CAS  Google Scholar 

  22. Pancheniak, E. R., & Soccol, C. R. (2005). B.CEPPA Curitiba, 23, 299–310.

    CAS  Google Scholar 

  23. Otero, M. C., Ocana, V. S., & Macias, E. N. M. (2004). Methods in Molecular Biology, 268, 435–440.

    Google Scholar 

  24. Rijnaarts, H. H. M., Norde, W., Bouwer, E. J., Lyklema, J., & Zehnder, A. J. B. (1993). Applied and Environmental Microbiology, 59, 3255–3265.

    CAS  Google Scholar 

  25. Gilbert, P., Evans, D. J., Evans, E., Duguid, I. G., & Brown, M. R. W. (1991). Journal of Applied Bacteriology, 71, 72–77.

    CAS  Google Scholar 

  26. Klein, G., Hallmann, C., Casas, L. A., Abad, J., Louwers, J., & Reuter, G. (2000). Journal of Applied Microbiology, 89, 815–824.

    Article  CAS  Google Scholar 

  27. Mathur, S., & Singh, R. (2005). International Journal of Food Microbiology, 105, 281–295.

    Article  CAS  Google Scholar 

  28. Belén Flórez, A., Delgado, S., & Mayo, B. (2005). Canadian Journal of Microbiology, 51, 51–58.

    Article  Google Scholar 

  29. Cerning, J., Bouillanne, C., Landon, M., & Desmazeaud, M. (1992). Journal of Dairy Science, 75, 692–699.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The corresponding author is grateful to the Department of Biotechnology (DBT), New Delhi, India for the financial support.

Author information

Authors and Affiliations

  1. National Institute for Interdisciplinary Science and Technology (formerly Regional Research Laboratory), CSIR, Trivandrum, 695 019, Kerala, India

    Ravindran Girija Aswathy, Bindhumol Ismail, Rojan Pappy John & Kesavan Madhavan Nampoothiri

Authors
  1. Ravindran Girija Aswathy
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bindhumol Ismail
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rojan Pappy John
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kesavan Madhavan Nampoothiri
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kesavan Madhavan Nampoothiri.

Additional information

R. G. Aswathy and B. Ismail contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Aswathy, R.G., Ismail, B., John, R.P. et al. Evaluation of the Probiotic Characteristics of Newly Isolated Lactic Acid Bacteria. Appl Biochem Biotechnol 151, 244–255 (2008). https://doi.org/10.1007/s12010-008-8183-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-008-8183-6

Keywords

Search

Navigation