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Culturable endophytic filamentous fungi from leaves of transgenic imidazolinone-tolerant sugarcane and its non-transgenic isolines

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Abstract

The diversity of endophytic filamentous fungi from leaves of transgenic imidazolinone-tolerant sugarcane plants and its isoline was evaluated by cultivation followed by amplified rDNA restriction analysis (ARDRA) of randomly selected strains. Transgenic and non-transgenic cultivars and their crop management (herbicide application or manual weed control) were used to assess the possible non-target effects of genetically modified sugarcane on the fungal endophytic community. A total of 14 ARDRA haplotypes were identified in the endophytic community of sugarcane. Internal transcribed spacer (ITS) sequencing revealed a rich community represented by 12 different families from the Ascomycota phylum. Some isolates had a high sequence similarity with genera that are common endophytes in tropical climates, such as Cladosporium, Epicoccum, Fusarium, Guignardia, Pestalotiopsis and Xylaria. Analysis of molecular variance indicated that fluctuations in fungal population were related to both transgenic plants and herbicide application. While herbicide applications quickly induced transient changes in the fungal community, transgenic plants induced slower changes that were maintained over time. These results represent the first draft on composition of endophytic filamentous fungi associated with sugarcane plants. They are an important step in understanding the possible effects of transgenic plants and their crop management on the fungal endophytic community.

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Acknowledgments

We thank Dr. Eugênio César Ulian (Centro de Tecnologia Canavieira) for transgenic and non-transgenic sugarcane plants and crop sites. This work was supported by FAPESP (Proc. no. 02/14143-3). We also thank CAPES for providing the fellowship to R.M.S.

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Authors and Affiliations

  1. Genetics Department, Escola Superior de Agricultura “Luiz de Queiroz”, University of São Paulo, Avenida Pádua Dias, 11, P.O. Box 83, Piracicaba, SP, 13400-970, Brazil

    Rodrigo Makowiecky Stuart, Aline Silva Romão, Aline Aparecida Pizzirani-Kleiner & João Lúcio Azevedo

  2. Laboratory of Molecular Biology and Microbial Ecology, NIB, University of Mogi das Cruzes, Avenida Dr. Cândido Xavier de Almeida Souza, 200, Mogi das Cruzes, SP, 08780-911, Brazil

    Welington Luiz Araújo

Authors
  1. Rodrigo Makowiecky Stuart
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  2. Aline Silva Romão
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  3. Aline Aparecida Pizzirani-Kleiner
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  4. João Lúcio Azevedo
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  5. Welington Luiz Araújo
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Correspondence to Welington Luiz Araújo.

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Communicated by Erko Stackebrandt.

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Stuart, R.M., Romão, A.S., Pizzirani-Kleiner, A.A. et al. Culturable endophytic filamentous fungi from leaves of transgenic imidazolinone-tolerant sugarcane and its non-transgenic isolines. Arch Microbiol 192, 307–313 (2010). https://doi.org/10.1007/s00203-010-0557-9

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  • DOI: https://doi.org/10.1007/s00203-010-0557-9

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