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Fungal endophytic community associated with Hevea spp.: diversity, enzymatic activity, and biocontrol potential

  • Environmental Microbiology - Research Paper
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Abstract

Plants of the genus Hevea present a great diversity of endophytic fungal species, which can provide bioactive compounds and enzymes for biotechnological use, and antagonist agents for plant disease biological control. The diversity of endophytic fungi associated with leaves of Hevea spp. clones in western Amazonia was explored using cultivation-based techniques, combined with the sequencing of the ITS rRNA-region. A total of 269 isolates were obtained, and phylogenetic analysis showed that they belong to 47 putative species, of which 24 species were unambiguous. The phylum Ascomycota was the most abundant (95.4%), with predominance of the genera Colletotrichum and Diaporthe, followed by the phylum Basidiomycota (4.6%), with abundance of the genera Trametes and Phanerochaete. Endophytic composition was influenced by the clones, with few species shared among them, and the greatest diversity was found in clone C44 (richness: 26, Shannon: 14,15, Simpson: 9.11). The potential for biocontrol and enzymatic production of endophytes has been investigated. In dual culture tests, 95% of the isolates showed inhibitory activity against C. gloeosporioides, and 84% against C. cassiicola. Efficient inhibition was obtained with isolates HEV158C and HEV255M (Cophinforma atrovirens and Polyporales sp. 2) for C. gloeosporioides, and HEV1A and HEV8B (Phanerochaete sp. 3 and Diaporthe sp. 4) for C. cassiicola. The endophytic isolates were positive for lipase (69.6%), amylase (67.6%), cellulase (33.3%), and protease (20.6%). The enzyme index ≥ 2 was found for amylase and lipase. The isolates obtained from rubber trees showed good antimicrobial and enzymatic potential, which can be tested in the future for use in the industry, and in the control of plant pathogens.

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Acknowledgements

The authors thank to Dr. Ewerton Cordeiro and Embrapa Amazônia Ocidental for collecting the Hevea samples and to CAPES for research support and for the scholarship of the first author.

Funding

This research was funded by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) project Pró-Amazônia n◦ 3287/13.

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Authors and Affiliations

  1. Universidade Federal do Amazonas, Programa de Pós-Graduação em Ciências Florestais e Ambientais, Manaus, Amazonas, Brazil

    Adriene de Oliveira Amaral & Jânia Lília da Silva Bentes

  2. Universidade Federal do Amazonas, Programa de Pós-Graduação em Agronomia Tropical, Manaus, Amazonas, Brazil

    Ana Francisca Tibúrcia Amorim Ferreira e Ferreira & Jânia Lília da Silva Bentes

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  1. Adriene de Oliveira Amaral
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  2. Ana Francisca Tibúrcia Amorim Ferreira e Ferreira
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  3. Jânia Lília da Silva Bentes
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Contributions

Conceptualization: Bentes, JLS. Investigation: Amaral, AO; Ferreira, AFTAF; and Bentes, JLS. Data curation: Ferreira, AFTAF; Amaral, AO; and Bentes, JLS. Writing: Amaral, AO, Ferreira, AFTAF; and Bentes, JLS. Funding: Bentes, JLS.

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Correspondence to Jânia Lília da Silva Bentes.

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de Oliveira Amaral, A., e Ferreira, A. & da Silva Bentes, J. Fungal endophytic community associated with Hevea spp.: diversity, enzymatic activity, and biocontrol potential. Braz J Microbiol 53, 857–872 (2022). https://doi.org/10.1007/s42770-022-00709-1

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