Abstract
Burkholderia pseudomallei, an infectious Gram-negative bacterium, is the causative pathogen of melioidosis. In the present study, a B. pseudomallei strain with mutation in the bsaQ gene, encoding a structural component of the type III secretion system (T3SS), was constructed. This bsaQ mutation caused a marked decrease in secretion of BopE effector and BipD translocator proteins into culture supernatant. The B. pseudomallei bsaQ mutant also exhibited decreased efficiencies of plaque formation, invasion into non-phagocytic cells and multinucleated giant cell (MNGC) development in a J774A.1 macrophage cell line. Co-localization of the bacteria and lysosome-associated membrane glycoprotein-1 (LAMP-1) containing vesicles suggested that defects in MNGC formation may result from the delayed ability of this B. pseudomallei mutant to escape from the vacuoles of macrophages.
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Abbreviations
- T3SS:
-
Type III secretion system
- BsaQ:
-
Burhkolderia secretion apparatus Q
- LAMP-1:
-
Lysosome-associated membrane glycoprotein-1
- MNGC:
-
Multinucleated giant cell
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Acknowledgments
This work was supported by the Siriraj Grant for Research and Development. V. Muangsombut is supported by Siriraj Graduate Thesis Scholarship. S. Suparak is supported by a postdoctoral grant from the Commission on Higher Education Thailand. We are grateful to M.P. Stevens (Compton Laboratory, UK), Dr. E.E. Galyov (Leicester University, UK) and Prof. R. Titball (DSTL, UK) for providing B. pseudomallei specific antisera and suggestions. We wish to thank E. Singsuksawat for RT-PCR analysis. We are also grateful to Dr. J. Cuccui for his kind help on editing the manuscript.
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Communicated by Sebastian Suerbaum.
Veerachat Muangsombut and Supaporn Suparak contributed equally to this work.
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Muangsombut, V., Suparak, S., Pumirat, P. et al. Inactivation of Burkholderia pseudomallei bsaQ results in decreased invasion efficiency and delayed escape of bacteria from endocytic vesicles. Arch Microbiol 190, 623–631 (2008). https://doi.org/10.1007/s00203-008-0413-3
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DOI: https://doi.org/10.1007/s00203-008-0413-3