Abstract
Melioidosis, a human infectious disease with a high mortality rate in many tropical countries, is caused by the pathogen Burkholderia pseudomallei (B. pseudomallei). The function of the B. pseudomallei sigma S (RpoS) transcription factor in survival during the stationary growth phase and conditions of oxidative stress is well documented. Besides the rpoS, bioinformatics analysis of B. pseudomallei genome showed the existence of two rpoN genes, named rpoN1 and rpoN2. In this study, by using the mouse macrophage cell line RAW264.7 as a model of infection, the involvement of B. pseudomallei RpoS and RpoN2 in the invasion, intracellular survival leading to the reduction in multinucleated giant cell (MNGC) formation of RAW264.7 cell line were illustrated. We have demonstrated that the MNGC formation of RAW264.7 cell was dependent on a certain number of intracellular bacteria (at least 5 × 104). In addition, the same MNGC formation (15%) observed in RAW264.7 cells infected with either B. pseudomallei wild type with multiplicity of infection (MOI) 2 or RpoN2 mutant (∆rpoN2) with MOI 10 or RpoS mutant (∆rpoS) with MOI 100. The role of B. pseudomallei RpoS and RpoN2 in the regulation of type III secretion system on bipB-bipC gene expression was also illustrated in this study.
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Funding
This work was supported by the TRIG project from the University of Medicine and Pharmacy at Ho Chi Minh City Vietnam and Graduate Program from Biochemistry Department, Faculty of Science, Mahidol University (for Duong Thi Hong Diep). This work was also supported by research grants from the Thailand Research Fund (BRG5680010).
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D.T.H. Diep: concept, acquisition, analysis, interpretation of data. L.B.Vong: acquisition, analysis, or interpretation of data. S. Tungpradabkul: concept, supervision. All authors prepared the manuscript and approved the version to be submitted.
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Diep, D.T.H., Vong, L.B. & Tungpradabkul, S. Function of Burkholderia pseudomallei RpoS and RpoN2 in bacterial invasion, intracellular survival, and multinucleated giant cell formation in mouse macrophage cell line. Antonie van Leeuwenhoek 117, 39 (2024). https://doi.org/10.1007/s10482-024-01944-2
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DOI: https://doi.org/10.1007/s10482-024-01944-2