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Novel lipoarabinomannan-like lipoglycan (CdiLAM) contributes to the adherence of Corynebacterium diphtheriae to epithelial cells

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Abstract

The genus Corynebacterium is part of the phylogenetic group nocardioform actinomycetes. Members of this group have a characteristic cell envelope structure composed primarily of branched long-chain lipids, termed mycolic acids, and a rich number of lipoglycans such as lipoarabinomanans (LAM) and lipomannans. In this study, we identified a novel LAM variant isolated from Corynebacterium diphtheriae named CdiLAM. The key structural features of CdiLAM are a linear α-1→6-mannan with side chains containing 2-linked α-D-Manp and 4-linked α-D-Araf residues. The polysaccharide backbone is linked to a phosphatidylinositol anchor. In contrast to the LAMs of other members of actinomycetales, CdiLAM presents an unusual substitution at position 4 of α-1→6-mannan backbone by α-D-Araf. Unlike the non-fimbrial adhesin 62–72p, CdiLAM did not function as a hemagglutinin to human red blood cells. Experimental evidences pointed to CdiLAM as an adhesin of C. diphtheriae to human respiratory epithelial cells, thereby, contributing to the pathogenesis of diphtheria.

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Acknowledgments

We are grateful to Dr. José Osvaldo Previato and Dr. Adriane Regina Todeschini for technical assistance with lipoglycan analysis and the Centro Nacional de Ressonância Mangnética Nuclear, UFRJ, Brasil, for the NMR facilities. This work was supported by a grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ), SR2-UERJ and Programa de Núcleo de Excelência (PRONEX) of the Brazilian Ministry of Science and Technology.

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Authors and Affiliations

  1. Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil

    L. O. Moreira

  2. Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil

    L. O. Moreira, A. L. Mattos-Guaraldi & A. F. B. Andrade

  3. Department of Infectious Diseases, St. Children’s Research Hospital, 332 North Lauderdale, Memphis, TN, 38105, USA

    L. O. Moreira

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  1. L. O. Moreira
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  2. A. L. Mattos-Guaraldi
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  3. A. F. B. Andrade
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Correspondence to L. O. Moreira.

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Communicated by Axel Brakhage.

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Moreira, L.O., Mattos-Guaraldi, A.L. & Andrade, A.F.B. Novel lipoarabinomannan-like lipoglycan (CdiLAM) contributes to the adherence of Corynebacterium diphtheriae to epithelial cells. Arch Microbiol 190, 521–530 (2008). https://doi.org/10.1007/s00203-008-0398-y

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  • DOI: https://doi.org/10.1007/s00203-008-0398-y

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