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Combined estrogen and ghrelin administration decreases expression of p27kip1 and proportion of isomyosin type I in the striated urethral and anal sphincters and levator ani of old ovariectomized rats

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Abstract

We compared estrogen and/or ghrelin effects on pelvic floor muscles in old versus young adult ovariectomized rats. Ovariectomized Fisher 344 rats (18 and 3 months old, n = 24 × 2) received 42 daily intraperitoneal 17-β estradiol (10 μg kg−1), ghrelin (2 μg kg−1), both, or vehicle (n = 6 × 4/group). Cytoplasmic p27kip1 expression and isomyosin I proportion in striated urethral and anal sphincters and levator ani were measured, respectively, by Western blot analysis and gel electrophoresis with immunohistochemistry of muscle ghrelin receptors and radioimmunoassay of circulating growth hormone. In young adult rats, estrogen significantly decreased cytoplasmic p27kip1 and isomyosin I signal intensities. In old rats, ghrelin and estrogen/ghrelin significantly decreased both intensities with greater estrogen/ghrelin effect. Ghrelin receptors were not immunostained in any muscle. Estrogen and/or ghrelin significantly increased or decreased, respectively, circulating growth hormone in old and young adult rats. Estrogen/ghrelin administration reversed pelvic floor muscle ageing changes in old ovariectomized rats through growth hormone production.

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Acknowledgments

This study was supported by a research grant from the Research Affairs, United Arab Emirates University, N 01-01-8-12/04. We would like to thank Dr. S. Benedict for technical assistance with radioimmunoassay of serum growth hormone.

Conflicts of interest

None.

Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates

    Diaa E. E. Rizk

  2. Department of Biology, Faculty of Science, United Arab Emirates University, Al Ain, United Arab Emirates

    Hazem A. Hassan

  3. Department of Biochemistry, United Arab Emirates University, Al Ain, United Arab Emirates

    Ahmed H. Al-Marzouqi

  4. Department of Pharmacology, United Arab Emirates University, Al Ain, United Arab Emirates

    Mohammed Shafiullah

  5. Department of Physiology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates

    Mohamed A. Fahim

  6. P.O. Box 17666, Al Ain, United Arab Emirates

    Diaa E. E. Rizk

Authors
  1. Diaa E. E. Rizk
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  2. Hazem A. Hassan
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  3. Ahmed H. Al-Marzouqi
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  4. Mohammed Shafiullah
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  5. Mohamed A. Fahim
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Corresponding author

Correspondence to Diaa E. E. Rizk.

Additional information

This article is dedicated to the memory of Dr. Hazem A. Hassan, Ph.D., Associate Professor of Biology and Director of the Masters in Environmental Science Program, United Arab Emirates University, who died during the preparation of the manuscript.

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Rizk, D.E.E., Hassan, H.A., Al-Marzouqi, A.H. et al. Combined estrogen and ghrelin administration decreases expression of p27kip1 and proportion of isomyosin type I in the striated urethral and anal sphincters and levator ani of old ovariectomized rats. Int Urogynecol J 19, 1363–1369 (2008). https://doi.org/10.1007/s00192-008-0638-3

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  • DOI: https://doi.org/10.1007/s00192-008-0638-3

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