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The effect of ovariectomy on biomarkers of urogenital ageing in old versus young adult rats

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Abstract

The aim of this study was to compare the effects of ageing and ovariectomy on biomarkers of urogenital ageing in old and young-adult rats. Fisher 344 rats (18- and 3-months-old, n = 6 × 2) underwent ovariectomy. Age-matched sham animals received no intervention (n = 6 × 2). One month later, biomarkers of urogenital ageing were evaluated (light microscopic count of urethral and anal canal submucosal blood vessels, Western blot analysis of urethral, and anal canal submucosal collagen I and III and cytoplasmic p27kip1 expression in the striated urethral and anal sphincters and levator ani and gel electrophoresis of isomyosin I proportion in these muscles) and compared in all groups (n = 24). All biomarkers of urogenital ageing studied were significantly increased in old compared to young-adult sham rats. Ovariectomy significantly increased these changes further in old versus young-adult rats with either smaller or larger differential effect than ageing compared to young-adult sham animals. Ovariectomy significantly exacerbates normative urogenital ageing changes in rats.

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References

  1. Tannenbaum C, Perrin L, Kuchel GA (2001) The aging pelvic floor. In: Crocos J, Schick E (eds) The urinary sphincter. Marcel Dekker, New York, pp. 175–181

    Google Scholar 

  2. Plas E, Daha LK (2004) Hormone replacement therapy for the aging bladder. In: Plas E, Pfluger H, Maier, Hubner W (eds) The aging bladder. Springer, Berlin Heidelberg New York, pp 137–154

    Google Scholar 

  3. Pfisterer MH, Griffiths DJ, Schaefer W, Resnick NM (2006) The effect of age on lower urinary tract function: a study in women. J Am Geriatr Soc 54:405–412

    Article  PubMed  Google Scholar 

  4. Cayzergues L, El-Djouher Y, Tabard SB, Jestin A, Blanchard P, Giuliano F et al (2005) Morphological study of the spinal motoneurons controlling the urethral sphincter of female rats: Role of androgens in a menopausal model. J Urol 173:1022–1026

    Article  PubMed  CAS  Google Scholar 

  5. Moalli PA, Nancy S, Howden NS, Lowder JL, Navarro J, Debes KM et al (2005) A rat model to study the structural properties of the vagina and its supportive tissues. Am J Obstet Gynecol 192:80–88

    Article  PubMed  Google Scholar 

  6. Russell B, Baumann M, Heidkamp MC, Svanborg A (1996) Morphometry of the aging female rat urethra. Int Urogynecol J 7:30–36

    Article  CAS  Google Scholar 

  7. Yiou R, Delmas V, Carmeliet P, Gherardi RK, Barlovatz-Meimon G, Chopin DK et al (2001) The pathophysiology of pelvic floor disorders: evidence from a histometric study of the perineum and a mouse model of rectal prolapse. J Anat 199:599–607

    Article  PubMed  CAS  Google Scholar 

  8. Souccar C, Goncalo MC, Buck HS, Lima-Landman MT, Lapa AJ (2005) Mild dystrophic damage in the androgen-sensitive levator ani muscle of the mdx mouse. Neuromuscul Disord 15:48–56

    Article  PubMed  Google Scholar 

  9. Piccone CM, Brazeau GA, McCormick KM (2005) Effect of oestrogen on myofiber size and myosin expression in growing rats. Exp Physiol 90:87–93

    Article  PubMed  CAS  Google Scholar 

  10. Vardy MD, Gardener TR, Cosman F, Scotti RJ, Mikhail MS, Preiss Bloom AO et al (2005) The effects of hormone replacement on the biochemical properties of the uterosacral and round ligaments in the monkey model. Am J Obstet Gynecol 192:1741–1751

    Article  PubMed  CAS  Google Scholar 

  11. Rizk DEE, Mensah-Brown EP, Chandranath SI, Ahmed I, Shafiullah M, Patel M et al (2003) Effects of ovariectomy and hormone replacement on collagen and blood vessels of the urethral submucosa of rats. Urol Res 31:147–151

    Article  PubMed  CAS  Google Scholar 

  12. Mensah-Brown EP, Rizk DEE, Patel M, Chandranath SI, Adem A (2004) Effects of ovariectomy and hormone replacement on submucosal collagen and blood vessels of the anal canal of rats. Colorectal Dis 6:481–487

    Article  PubMed  CAS  Google Scholar 

  13. Lluel P, Palea S, Barras M, Grandadam F, Heudes D, Bruneval P et al (2000) Functional and morphological modifications of the urinary bladder in aging female rats. Am J Physiol 278:R964–R972

    CAS  Google Scholar 

  14. Rizk DEE, Hassan HA, Ramadan GA, Shafiullah M, Fahim MA (2005) Estrogen and ghrelin increase the number of submucosal urethral and anal canal blood vessels in ovariectomized rats. Urology 66:1343–1348

    Article  PubMed  Google Scholar 

  15. Moalli PA, Talarico LC, Sung VW, Kingensmith WL, Shand SH, Meyn LA et al (2004) Impact of menopause on collagen subtypes in the arcus tendinous fascia pelvis. Am J Obstet Gynecol 190:620–627

    Article  PubMed  CAS  Google Scholar 

  16. Bukovsky A, Copas P, Caudle MR, Cekanova M, Dassanayake T, Asbury B et al (2004) Abnormal expression of p27kip1 protein in levator muscle of aging women with pelvic floor disorders—a relationship to the cellular differentiation and degeneration. BMC Clin Pathol 1:4

    Article  Google Scholar 

  17. Rizk DEE, Al-Marzouqi AH, Hassan HA, Fahim MA (2006) Estrogen and ghrelin decrease cytoplasmic expression of p27kip1, a cellular marker of ageing, in the striated anal sphincter and levator muscle of ovariectomized rats. Int Urogynecol J (in press)

  18. Kojima M, Kangawa K (2006) Drug insight: the functions of ghrelin and its potential as a multitherapeutic hormone. Nature Clin Pract Endocrinol Metab 2:80–88

    Article  CAS  Google Scholar 

  19. Chu MC, Cosper P, Orio F, Carmina E, Lobo RA (2006) Insulin resistance in postmenopausal women with metabolic syndrome and the measurements of adiponectin, leptin, resistin, and ghrelin. Am J Obstet Gynecol 194:100–104

    Article  PubMed  CAS  Google Scholar 

  20. Lelbach A, Feher J, Szekacs B (2006) The molecular biology of ageing-therapeutic interventions? Orv Hetil 147:441–448

    PubMed  Google Scholar 

  21. Jundt K, Kiening M, Fischer P, Bergauer F, Rauch E, Janni W et al (2005) Is the histomorphological concept of the female pelvic floor and its changes due to age and vaginal delivery correct? Neurourol Urodyn 24:44–50

    Article  PubMed  Google Scholar 

  22. Kadi F, Karlsson C, Larsson B, Eriksson J, Larval M, Billing H et al (2002) The effects of physical activity and estrogen treatment on rat fast and slow skeletal muscles following ovariectomy. J Muscle Res Cell Motil 23:335–339

    Article  PubMed  CAS  Google Scholar 

  23. Dimpfl T, Jaeger C, Mueller-Felber W, Anthuber C, Hirsch A, Brandmaier R et al (1998) Myogenic changes of the levator ani muscle in premenopausal women: the impact of vaginal delivery and age. Neurourol Urodyn 17:197–205

    Article  PubMed  CAS  Google Scholar 

  24. Zhu L, Lang JH, Chen J, Chen J (2005) Morphologic study on levator ani muscle in patients with pelvic organ prolapse and stress urinary incontinence. Int Urogynecol J 16:401–414

    Article  Google Scholar 

  25. Markou A, Duka T, Prelevic GM (2005) Estrogens and brain function. Hormones 4:9–17

    PubMed  Google Scholar 

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Acknowledgment

This study was supported by a research grant from the Research Affairs, United Arab Emirates University, N 01-01-8-12/04. We would like to thank Mr. S. Daoud and Mr. M. Shafiullah, respectively, for technical assistance with processing of tissue samples and the ovariectomy procedure.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Faculty of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al-Ain, United Arab Emirates

    Diaa E. E. Rizk & Layla S. Al-Ghafri

  2. Department of Physiology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al-Ain, United Arab Emirates

    Mohamed A. Fahim

  3. Department of Biology, Faculty of Science, United Arab Emirates University, Al-Ain, United Arab Emirates

    Hazem A. Hassan & Gaber A. Ramadan

  4. Department of Biochemistry, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al-Ain, United Arab Emirates

    Ahmed H. Al-Marzouqi & Soha S. Al-Kedrah

Authors
  1. Diaa E. E. Rizk
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  2. Mohamed A. Fahim
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  3. Hazem A. Hassan
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  4. Ahmed H. Al-Marzouqi
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  5. Gaber A. Ramadan
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  6. Soha S. Al-Kedrah
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  7. Layla S. Al-Ghafri
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Corresponding author

Correspondence to Diaa E. E. Rizk.

Additional information

Dedication: This article is dedicated to the memory of Dr. Hazem A. Hassan, Ph.D., Associate Professor of Biology and Director of the Masters in Environmental Science Program, United Arab Emirates University, who died during the preparation of the manuscript.

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Rizk, D.E.E., Fahim, M.A., Hassan, H.A. et al. The effect of ovariectomy on biomarkers of urogenital ageing in old versus young adult rats. Int Urogynecol J 18, 1077–1085 (2007). https://doi.org/10.1007/s00192-006-0278-4

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  • DOI: https://doi.org/10.1007/s00192-006-0278-4

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