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Rapid Detection of Sublethal Toxicity Using Locomotor Activity of Rainbow Trout Juveniles

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Abstract

This study examined changes in locomotor activity of the rainbow trout (Oncorhynchus mykiss) juveniles exposed to sublethal concentrations of hexavalent chromium (Cr6+) (as a single pollutant) and landfill leachate (as an complex mixture of mainly organic compounds, including trace amounts of metals). Fish were first examined for baseline behavior patterns for 3 h to determine the appropriate duration of acclimation and the control level. The average velocity of fish was found to be the most informative among other endpoints throughout the 3 h long acclimation. Under the effect of both test substances, fish locomotor activity significantly increased after 5 min reaching maximum values after 10 min of exposure. The juveniles exposed to leachate were more responsive than those exposed to Cr6+. However, in this study we did not succeed in identifying pollutants from the elicited fish behavioral response patterns.

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References

  • Alkassasbeh JYM, Heng LH, Surif S (2009) Toxicity testing and the effect of landfill leachate in Malaysia on behavior of common carp (Cyprinus carpio L., 1758; Pisces, Cyprinidae). Am J Envi Sci 5(3):209–217

    Article  CAS  Google Scholar 

  • Ašmonaitė G, Boyer S, Souza KB, Wassmur B, Sturve J (2016) Behaviuoral toxicity assessment of silver ions and nanoparticles on zebrafish using a locomotion profiling approach. Aquat Toxicol 173:143–153

    Article  Google Scholar 

  • ASTM (2012) Standard guide for measurement of behavior during fish toxicity tests. ASTM E1711-12, International, West Conshohocken, pp 1–15

    Google Scholar 

  • ASTM (2014) Standard guide for conducting acute toxicity tests on test materials with fishes, macroinvertebrates, and amphibians. ASTM International, West Conshohocken

    Google Scholar 

  • Bae MJ, Park YS (2014) Biological early warning system based on the responses of aquatic organisms to disturbances: a review. Sci Total Environ 466–467:635–649

    Article  Google Scholar 

  • Barton AB (2002) Stress in fishes: a diversity of responses with particular reference to changes in circulating corticosteroids. Integr Comp Biol 42:517–525

    Article  CAS  Google Scholar 

  • Diamond JM, Parson MJ, Gruber D (1990) Rapid detection of sublethal toxicity using fish ventilator behavior. Environ Toxicol Chem 9(1):3–11

    Article  Google Scholar 

  • EPA (2002). Methods for measuring the acute toxicity of effluents and receiving waters to freshwater and marine organisms, 5th edn. U. S. Environmental Protection Agency, Washington, D.C., pp 1–275

    Google Scholar 

  • Ghosh P, Thakur IS, Kaushikc A (2017) Bioassays for toxicological risk assessment of landfill leachate: a review. Ecotoxicol Environ Saf 141:259–270

    Article  CAS  Google Scholar 

  • Gonҫalves R, Scholze M, Ferreira AM, Martins M, Correia AD (2008) The joint effect of polycyclic aromatic hydrocarbons on fish behavior. Environ Res 108:205–213

    Article  Google Scholar 

  • Hellou J (2011) Behavioural ecotoxicology, an “early warning” signal to assess environment quality. Environ Sci Pollut Res 18:1–11

    Article  CAS  Google Scholar 

  • Huang Y, Zhang J, Xiaobo H, Huang T (2014) The use of zebrafish (Danio rerio) behavioral responses in identifying sublethal exposures of deltamethrin. Int J Environ Res Public Health 11:3650–3660

    Article  Google Scholar 

  • ISO 10304-1:2007 Water quality—determination of dissolved anions by liquid chromatography of ions—part 1: determination of bromide, chloride, fluoride, nitrate, nitrite, phosphate and sulfate, ISO, the International Organization for Standardization

  • ISO 9963-1:1994 Water quality—determination of alkalinity—part 1: determination of total and composite alkalinity ISO, the International Organization for Standardization

  • ISO 14911:1998 Water quality—determination of dissolved Li+, Na+, NH4+, K+, Mn2+, Ca2+, Mg2+, Ba2+ using ion chromatography—method for water and waste water, ISO

  • ISO 10523:2008 Water quality—determination of pH, ISO, the International Organization for Standardization

  • ISO 8467:1993 Water quality—determination of permanganate index, ISO, the International Organization for Standardization

  • ISO 7888:1985 Water quality—determination of electrical conductivity, ISO, the International Organization for Standardization

  • ISO 15586:2003 Water quality—determination of trace elements using atomic absorption spectrometry with graphite furnace, ISO, the International Organization for Standardization

  • Kienzler A, Bopp SK, Linden SVD, Berggen E, Worth A (2016) Regulatory assessment of chemical mixtures: requirements current approaches and future perspectives. Reg Toxicol Pharmacol 80:321–334

    Article  CAS  Google Scholar 

  • Lushchak V (2011) Environmentally induced oxidative stress in aquatic animals. Aquat Toxicol 101:13–30

    Article  CAS  Google Scholar 

  • Melvin SD, Petit MA, Duvignacq MC, Sumpter JP (2017) Towards improved behavioural testing in aquatic toxicology: acclimation and observation times are important factors when designing behavioural tests with fish. Chemosphere 180:430–436

    Article  CAS  Google Scholar 

  • Michalec FG, Holzner M, Menu D, Hwang JS, Souissi S (2013) Behavioral responses of the estuarine calanoid copepod Eurytemora affinis to sub-lethal concentrations of waterborne pollutants. Aquat Toxicol 138–139:129–138

    Article  Google Scholar 

  • Papadakis VM, Glaropoulos A, Kentouri M (2014) Sub-second analysis of fish behavior using a novel computer-vision system. Aquacult Eng 62:36–41

    Article  Google Scholar 

  • Sandheinrich M (2014) Role of behavior in ecotoxicology. In: Newman CM (ed) Fundamentals of ecotoxicology—the science of pollution, 4th edn. CRC Press, New York, pp 265–267

    Google Scholar 

  • Scott RG, Sloman AK (2004) The effects of environmental pollutants on complex fish behavior integrating behavioural and physiological indicators of toxicity. Aquat Toxicol 68:369–392

    Article  CAS  Google Scholar 

  • Svecevičius G (2007) Avoidance response of rainbow trout Oncorhynchus mykiss to hexavalent chromium solutions. Bull Environ Contam Toxicol 79:596–600

    Article  Google Scholar 

  • Svecevičius G (2012) The acute and behavioral effects of a copper-nickel mixture on roach Rutilus rutilus. Bull Environ Contam Toxicol 89:147–151

    Article  Google Scholar 

  • Svecevičius G, Kazlauskienė N, Taujanskis E (2012) The acute and behavioral response of a copper-nickel mixture on roach Rutilus rutilus. Bull Environ Contam Toxicol 88(1):1–5

    Article  Google Scholar 

  • Tierney KB, Baldwin DH, Hara TJ, Ross PS, Scholz NL, Kennedy CJ (2010) Olfactory toxicity in fishes. Aquat Toxicol 96:2–26

    Article  CAS  Google Scholar 

  • Van der Schalie WH, Shedd TR, Widder MW, Brennan LM (2004) Response characteristics of an aquatic biomonitor used for rapid toxicity detection. J Appl Toxicol 24(5):387–394

    Article  Google Scholar 

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Acknowledgements

This work was supported by the Open Access to research infrastructure of the Nature Research Center and National Cancer Institute under the Lithuanian open access network initiative. This work was funded by the Research Council of Lithuania, Project No. MIP-108/2015.

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Authors and Affiliations

  1. Laboratory of Ecology and Physiology of Hydrobionts of Nature Research Centre, Akademijos St. 2, 08412, Vilnius, Lithuania

    Tomas Makaras, Gintaras Svecevičius, Nijolė Kazlauskienė & Danguolė Montvydienė

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  1. Tomas Makaras
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  2. Gintaras Svecevičius
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  3. Nijolė Kazlauskienė
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  4. Danguolė Montvydienė
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Correspondence to Tomas Makaras.

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Gintaras Svecevičius—Deceased May 2016.

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Makaras, T., Svecevičius, G., Kazlauskienė, N. et al. Rapid Detection of Sublethal Toxicity Using Locomotor Activity of Rainbow Trout Juveniles. Bull Environ Contam Toxicol 100, 221–227 (2018). https://doi.org/10.1007/s00128-017-2244-x

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  • DOI: https://doi.org/10.1007/s00128-017-2244-x

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