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Survival, Reproduction, Avoidance Behavior and Oxidative Stress Biomarkers in the Earthworm Octolasion cyaneum Exposed to Glyphosate

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Abstract

The massive use of glyphosate (GLY) in several countries has increased the interest in investigating its potential adverse effects in non-target organisms. The aim of the present study was to assess the potential effects in survival and reproduction; avoidance behavior and oxidative stress under short-term (48 h) and subchronic exposures (28 days) to GLY in the earthworm Octolasion cyaneum. After 48 h no significant changes in the behavior was observed. In addition, a lower catalase activity at 498 μg GLY kg−1 dry soil section relative to earthworms from the control section was obtained. After 28 days of exposure inhibition of glutathione S-transferase activity was observed at 535 μg GLY kg−1 dry soil while no changes in the other endpoints were detected. These results indicate that environmentally relevant concentrations of GLY (up to 996 µg GLY kg−1 dry soil) did not exert a toxic effect to O. cyaneum.

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Acknowledgments

Thank you to N. Clemente for the statistical analysis. This work was supported by CONICET (PIP 112-201-101-01084) and UNMdP (EXA 608/12 and AGR 390/12).

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Authors and Affiliations

  1. Unidad Integrada Facultad de Ciencias Agrarias (UNMdP) - EEA INTA Balcarce, C.C. 276 (7620), Balcarce, Argentina

    Carla Salvio, Sergio Rafael & Pablo L. Manetti

  2. Laboratorio de Ecotoxicología, Instituto de Investigaciones Marinas y Costeras, CONICET – Universidad Nacional de Mar del Plata, Funes 3350, 7600 Mar del Plata, Buenos Aires, Argentina

    Mirta L. Menone & Fernando G. Iturburu

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  1. Carla Salvio
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  2. Mirta L. Menone
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  3. Sergio Rafael
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Correspondence to Mirta L. Menone.

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Salvio, C., Menone, M.L., Rafael, S. et al. Survival, Reproduction, Avoidance Behavior and Oxidative Stress Biomarkers in the Earthworm Octolasion cyaneum Exposed to Glyphosate. Bull Environ Contam Toxicol 96, 314–319 (2016). https://doi.org/10.1007/s00128-015-1700-8

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