Abstract
In this study, we compared the performance as screening tools of two yeast-based recombinant receptor-reporter gene bioassays with a commercial ELISA kit for measurement of total estrogens. For WWTP effluents there was a very good correlation between the measured total estrogen concentrations (ELISA) and estrogenic activity by the hERα bioassay (r2 = 0.93), but not for the medERα bioassay (r2 = 0.50). For freshwater samples, the correlations between bioassay response and ELISA ES measurments were very good (r2 > 0.95). There was no correlation between bioassay response and ELISA ES measurments for estuarine samples.
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Acknowledgments
The research was primarily supported by the Department of Primary Industries (Project #06889, 08162), with additional assistance from Melbourne Water and the Victorian Water Trust (Project #33 V-4000). The authors confirm that they received no funding from, nor have any financial ties with the manufacturer of the ELISA kit, nor does its use in this study imply any endorsement of the kit by the authors or their respective organisations. The project team gives its thanks to Kathryn Hassell, Kavitha Chinathamby, Trevor Theodoropoulos and Adam Wightwick for assistance with collection of samples, and all the Victorian water authorities, who, in one way or another, contributed to the success of this project.
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Allinson, M., Shiraishi, F. & Allinson, G. A Comparison of Recombinant Receptor-Reporter Gene Bioassays and a Total Estrogen Enzyme Linked Immunosorbent Assay for the Rapid Screening of Estrogenic Activity in Natural and Waste Waters. Bull Environ Contam Toxicol 86, 461–464 (2011). https://doi.org/10.1007/s00128-011-0251-x
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DOI: https://doi.org/10.1007/s00128-011-0251-x