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Transformation of a Texas cotton cultivar by using Agrobacterium and the shoot apex

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Abstract

 Transgenic cotton (Gossypium hirsutum L.) plants of a Texas cultivar CUBQHRPIS were obtained using Agrobacterium-mediated transformation coupled with the use of shoot-apex explants. After inoculation with A. tumefaciens strain LBA 4404 containing the pBI121 plasmid, regeneration of primary plants was carried out in a medium containing kanamycin (100  mg l-1). Progeny obtained by selfing were germinated in the greenhouse and selected for expression of the T-DNA marker gene encoding neomycin phospho-transferase II (NPTII) by painting kanamycin (2%) on the leaves. Plants that survived the leaf painting were analyzed by DNA blots. Evidence for integration of the transgene (GUS) was observed in two successive generations from the regenerants (T0). The transformed plants appeared to have more than one copy of the T-DNA.

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Authors and Affiliations

  1. Department of Soil and Crop Sciences, Texas A&M University, College Station, Texas 77843, USA Fax: +1-409-862-4301 E-mail: rsmith@tamu.edu, , , , , , US

    C. Zapata, S. H. Park, K. M. El-Zik & R. H. Smith

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  1. C. Zapata
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  2. S. H. Park
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  3. K. M. El-Zik
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  4. R. H. Smith
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Received: 1 June 1998 / Accepted: 28 July 1998

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Zapata, C., Park, S., El-Zik, K. et al. Transformation of a Texas cotton cultivar by using Agrobacterium and the shoot apex. Theor Appl Genet 98, 252–256 (1999). https://doi.org/10.1007/s001220051065

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  • DOI: https://doi.org/10.1007/s001220051065

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