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Recovery of transgenic rice plants expressing the rice dwarf virus outer coat protein gene (S8)

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Abstract

 The coding region of the eighth largest segment (S8) of the rice dwarf virus (RDV) was obtained from a RDV Fujian isolate. It was then cloned into pTrcHisA for expression in E. coli and into vector pE3 for plant transformation. By using callus derived from mature rice embryos as the target tissue, we obtained regenerated rice plants after bombardment of the former with plasmid pE3R8 containing the RDV S8 gene and the marker gene neomycin phosphotransferase (NPT II). Southern blotting confirmed the integration of the RDV S8 gene into the rice genome. The expression of the outer coat protein in both E. coli and rice plants was confirmed by western blotting. The recovery of transgenic rice plants expressing S8 gene is an important step towards studying the function of the RDV genes and obtaining RDV-resistant rice plants.

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Authors and Affiliations

  1. The National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing 100871, China, , , , , , CN

    H. H. Zheng, Y. Li, Z. H. Yu, W. Li, M. Y. Chen, X. T. Ming & Z. L. Chen

  2. Federal Biological Research Centre for Agriculture and Forestry, Institute of Biochemistry and Plant Virology, D-38104 Braunschweig, Germany, , , , , , DE

    R. Casper

Authors
  1. H. H. Zheng
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  2. Y. Li
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  3. Z. H. Yu
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  4. W. Li
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  5. M. Y. Chen
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  6. X. T. Ming
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  7. R. Casper
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  8. Z. L. Chen
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Received: 1 March 1996 / Accepted: 2 August 1996

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Zheng, H., Li, Y., Yu, Z. et al. Recovery of transgenic rice plants expressing the rice dwarf virus outer coat protein gene (S8). Theor Appl Genet 94, 522–527 (1997). https://doi.org/10.1007/s001220050446

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  • DOI: https://doi.org/10.1007/s001220050446

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