Abstract
Molecular markers linked to resistance genes are useful to facilitate the introgression of one or more of these genes in breeding materials. Following the approach of bulked segregant analysis, RAPD markers linked to resistance genes against beet necrotic yellow vein virus were identified in the four Beta accessions Holly-1–4, R104, R128 and WB42. Two primers were found which generate RAPD markers tightly linked to resistance in segregating families of Holly-1–4, R104 and R128, indicating that the resistance genes in these accessions might be situated at the same locus. Other, specific, primers were identified which generate RAPD markers linked to resistance in each of these accessions. Short-range maps were established around the resistance locus in these accessions. For WB42, RAPD markers were only identified at a relatively large distance from the resistance gene. Conversion of three RAPD primers of Holly-1–4, R104 and R128 into STS primers resulted in STS markers which can be readily used for marker-assisted selection in breeding programmes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Asher MJC (1993) Rhizomania. In: Cook DA, Scott RK (eds) The sugar beet crop. Chapman and Hall, London, pp 311–346
Baker H, Solomon-Blackburn RM, McNicol JW, Bradshaw JE (1994) Resistance to potato leaf roll virus multiplication in potato is under major gene control. Theor Appl Genet 88:754–758
Bürcky K, Büttner G (1985) Ansätze Zur Selektion rizomaniatoleranter Zuckerrüben während der Jugendentwicklung - I. Zuckerind 110:997–1000
Del Sal G, Manfioletti G, Schneider C (1988) A one-tube plasmid DNA mini-preparation suitable for sequencing. Nucleic Acids Res 16:9878
Fraser RSS (1990) The genetics of resistance to plant viruses. Annu Rev Phytopathol 28:179–200
Giunchedi L, DeBiaggi M, Poggi Pollini C (1985) Evaluation of ELISA technique for the screening of rhizomania-tolerant sugar beet genotypes. In: Proc 48th IIRB Winter Cong, Brussels, Belgium, pp 385–390
Jansen RC (1993) Maximum likelihood in a generalized linear finite mixture model by using the EM algorithm. Biometrics 49:227–231
Jansen RC (1994) Maximum likelihood in a finite mixture model by exploiting the GLM facilities of Genstart. Genstat Newslett 30:25–27
Johansson E (1985) Rhizomania in sugar beet — a threat to beet growing that can be overcome by plant breeding. Sver Utsädesförenings Tidskrift 95:115–121
Jung C, Pillen K, Frese L, Fähr S, Melchinger AE (1993) Phylogenetic relationships between cultivated and wild species of the genus Beta revealed by DNA “fingerprinting”. Theor Appl Genet 86:449–457
Lewellen RT (1988) Selection for resistance to rhizomania in sugar beet (abstract). 5th Int Cong Plant Pathol, Kyoto, Japan, pp 455
Lewellen RT, Biancardi E (1990) Breeding and performance of rhizomania-resistant sugar beet In: Proc 53rd IIRB Winter Cong, Brussels, Belgium, pp 69–87
Lewellen RT, Skoyen IO, Erichsen AW (1987) Breeding sugar beet for resistance to rhizomania: evaluation of host-plant reactions and selection for and inheritence of resistance. In: Proc 50th IIRB Winter Cong, Brussels, Belgium, pp 139–156
Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832
Olson M, Hood L, Cantor C, Botstein D (1989) A common language for physical mapping of the human genome. Science 245:1434–1435
Paul H, Henken B, Alderlieste MFJ (1992) A greenhouse test for screening sugar beet (Beta vulgaris) for resistance to beet necrotic yellow virus (BNYVV). Neth J Plant Pathol 98:65–75
Paul H, Henken B, Scholten OE, De Bock ThSM, Lange W (1993) Variation in the level of infection with Polymyxa betea and its effect on infection with beet necrotic yellow vein virus in beet accession of the sections Beta and Corollinae. In: Proc 2nd Symp Int Working Group on Plant Viruses with Fungal Vectors, Montreal, Canada, pp 133–136
Richard-Molard MS (1985) Rhizomania: a word wide danger to sugar beet. Span 28:92–94
Schlösser E (1988) Epidemiology and management of Polymyxa betae and beet necrotic yellow vein virus. In: Cooper JI, Asher MJC(eds) Viruses with fungal vectors, developments in applied biology 2. Association of Applied Biologists, Wellesbourne, pp 281–292
Scholten OE, Jansen RC, Keizer LCP, De Bock ThSM, Lange W (1996) Major genes for resistance to beet necrotic yellow vein virus (BNYVV) in Beta vulgaris. Euphytica 91:331–339
Stam P, Van Ooijen JW (1995) Joinmap (tm) version 2.0: Software for the calculation of genetic linkage maps. CPRO-DLO, Wageningen
Tamada T (1975) Beet necrotic yellow vein virus. CMI/AAB Descriptions of Plant Viruses, no. 144
Vanderbeek JG, Verkerk R, Zabel P, Lindhout P (1992) Mapping strategy for resistance genes in tomato based on RFLPs between cultivars-Cf9 (Resistance to Cladosporium fulvum) on chromosome-1. Theor Appi Genet 84:106–112
Van Ooijen JW (1994) A computer program for drawing genetic linkage maps. J. Hered 85:66
Van Ooijen JW, Malipaard C (1995) MapQTL (tm) version 3.0: software for the calculation of QTL positions on genetic maps. CPRO-DLO, Wageningen
Welsh J, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18:7213–7218
Whitney ED (1986) Correlations among greenhouse tests and between field and greenhouse evaluations for beet necrotic yellow vein virus (BNYVV) resistance in Beta maritima (abstract). Phytopathology 76:1074
Whitney ED (1989) Identification, distribution, and testing for resistance to rhizomania in Beta maritima. Plant Dis 73:287–290
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535
Author information
Authors and Affiliations
Additional information
Communicated by G. Wenzel
This research was carried out as part of a PhD study by O.E.S. at the Graduate School for Experimental Plant Sciences, Department of Virology, Wageningen Agricultural University Communicated by G. Wenzel
Rights and permissions
About this article
Cite this article
Scholten, O.E., Klein-Lankhorst, R.M., Esselink, D.G. et al. Identification and mapping of random amplified polymorphic DNA (RAPD) markers linked to resistance against beet necrotic yellow vein virus (BNYVV) in Beta accessions. Theoret. Appl. Genetics 94, 123–130 (1997). https://doi.org/10.1007/s001220050390
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s001220050390