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TdPm60 identified in wild emmer wheat is an ortholog of Pm60 and constitutes a strong candidate for PmG16 powdery mildew resistance

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A Correction to this article was published on 07 July 2021

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Abstract

Key message

We identified TdPm60 alleles from wild emmer wheat (WEW), an ortholog of Pm60 from T. urartu, which constitutes a strong candidate for PmG16 mildew resistance. Deployment of PmG16 in Israeli modern bread wheat cultivar Ruta improved the resistance to several local Bgt isolates.

Abstract

Wild emmer wheat (WEW), the tetraploid progenitor of durum and bread wheat, is a valuable genetic resource for resistance to powdery mildew fungal disease caused by Blumeria graminis f. sp. tritici (Bgt). PmG16 gene, derived from WEW, confers high resistance to most tested Bgt isolates. We mapped PmG16 to a 1.4-cM interval between the flanking markers uhw386 and uhw390 on Chromosome 7AL. Based on gene annotation of WEW reference genome Zavitan_V1, 34 predicted genes were identified within the ~ 3.48-Mb target region. Six genes were annotated as associated with disease resistance, of which TRIDC7AG077150.1 was found to be highly similar to Pm60, previously cloned from Triticum urartu, and resides in the same syntenic region. The functional molecular marker (FMM) for Pm60 (M-Pm60-S1) co-segregated with PmG16, suggesting the Pm60 ortholog from WEW (designated here as TdPm60) as a strong candidate for PmG16. Sequence alignment identified only eight SNPs that differentiate between TdPm60 and TuPm60. Furthermore, TdPm60 was found to be present also in the WEW donor lines of the powdery mildew resistance genes MlIW172 and MlIW72, mapped to the same region of Chromosome 7AL as PmG16, suggesting that TdPm60 constitutes a candidate also for these genes. Furthermore, screening of additional 230 WEW accessions with Pm60 specific markers revealed 58 resistant accessions from the Southern Levant that harbored TdPm60, while none of the susceptible accessions showed the presence of this gene. Deployment of PmG16 in Israeli modern bread wheat cultivar Ruta conferred resistance against several local Bgt isolates.

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Acknowledgements

Y.L. was supported by a fellowship provided by the Planning and Budgeting Committee (PBC) of the Israel Council for Higher Education for Outstanding Post-doctoral Fellows from China and India—from 2018 to 2021. Z.W. was supported by the China Scholarship Council for financial support and the University of Haifa Graduate School. The authors wish to thank Dr. Meng Xu for the development of the geographic distribution map, Dr. Tamar Kis-papo and Souad Khalifa for their technical support, Dr. Imad Shams and Tamar Lotan Labs for assistance in microscopy works, and Dr. Rajib Roychowdhury for critical reading and English editing of the manuscript.

Funding

This study was supported by the Israel Science Foundation, grant number 1719/08 and 1366/18.

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Author notes

  1. Zhen-Zhen Wei

    Present address: Department of Agronomy, the Key Laboratory of Crop Germplasm Resource of Zhejiang Province, Zhejiang University, Hangzhou, 310058, Zhejiang, China

  2. Andrii Fatiukha

    Present address: Crop Developmental Centre and Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK, S7N 5A8, Canada

  3. Yinghui Li and Zhen-Zhen Wei contributed equally to this work.

Authors and Affiliations

  1. Institute of Evolution, University of Haifa, Mt. Carmel, 3498838, Haifa, Israel

    Yinghui Li, Zhen-Zhen Wei, Andrii Fatiukha, Samidha Jaiwar, Hanchao Wang, Samiha Hasan, Hanan Sela, Tamar Krugman & Tzion Fahima

  2. The Department of Evolutionary and Environmental Biology, University of Haifa, Mt. Carmel, 3498838, Haifa, Israel

    Yinghui Li, Zhen-Zhen Wei, Andrii Fatiukha, Samidha Jaiwar, Hanchao Wang, Samiha Hasan & Tzion Fahima

  3. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China

    Zhiyong Liu

  4. University of Chinese Academy of Sciences, Beijing, 100049, China

    Zhiyong Liu

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Contributions

TF, YL, and ZW conceived and designed the study; ZW, AF, and YL developed the critical RILs; AF, ZW, and YL performed data analysis of genotyping by 15 K wheat SNP arrays of Trait Genetics®; HW, ZW, SJ, and YL were involved in marker development and analysis; YL, ZW, and SJ done microscopic observations; ZW, YL, SH, and SJ done phenotyping; YL and SJ contributed to sequencing; TK and YL performed germplasm selection; AF, YL, and HS done bioinformatic analysis of Pm60 locus; YL and TF wrote the manuscript; AF, HS, ZW, TK, and TF reviewed and edited the manuscript; TF was involved in coordination and funding acquisition.

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Correspondence to Tzion Fahima.

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Li, Y., Wei, ZZ., Fatiukha, A. et al. TdPm60 identified in wild emmer wheat is an ortholog of Pm60 and constitutes a strong candidate for PmG16 powdery mildew resistance. Theor Appl Genet 134, 2777–2793 (2021). https://doi.org/10.1007/s00122-021-03858-3

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