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Discovery and introgression of the wild sunflower-derived novel downy mildew resistance gene Pl 19 in confection sunflower (Helianthus annuus L.)

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A new downy mildew resistance gene, Pl 19 , was identified from wild Helianthus annuus accession PI 435414, introduced to confection sunflower, and genetically mapped to linkage group 4 of the sunflower genome.

Abstract

Wild Helianthus annuus accession PI 435414 exhibited resistance to downy mildew, which is one of the most destructive diseases to sunflower production globally. Evaluation of the 140 BC1F2:3 families derived from the cross of CMS CONFSCLB1 and PI 435414 against Plasmopara halstedii race 734 revealed that a single dominant gene controls downy mildew resistance in the population. Bulked segregant analysis conducted in the BC1F2 population with 860 simple sequence repeat (SSR) markers indicated that the resistance derived from wild H. annuus was associated with SSR markers located on linkage group (LG) 4 of the sunflower genome. To map and tag this resistance locus, designated Pl 19 , 140 BC1F2 individuals were used to construct a linkage map of the gene region. Two SSR markers, ORS963 and HT298, were linked to Pl 19 within a distance of 4.7 cM. After screening 27 additional single nucleotide polymorphism (SNP) markers previously mapped to this region, two flanking SNP markers, NSA_003564 and NSA_006089, were identified as surrounding the Pl 19 gene at a distance of 0.6 cM from each side. Genetic analysis indicated that Pl 19 is different from Pl 17 , which had previously been mapped to LG4, but is closely linked to Pl 17 . This new gene is highly effective against the most predominant and virulent races of P. halstedii currently identified in North America and is the first downy mildew resistance gene that has been transferred to confection sunflower. The selected resistant germplasm derived from homozygous BC2F3 progeny provides a novel gene for use in confection sunflower breeding programs.

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Acknowledgments

The authors would like to thank Dr. Loren Rieseberg for providing access to the Sunflower Genome Data Repository. We also thank Drs. Zhao Liu and Steven Xu for critical review of the manuscript, Angelia Hogness for technical assistance, and Michelle Gilley for providing some downy mildew isolates of different races. ZWZ was supported by China Scholarship Council. This project was supported by the USDA-ARS CRIS Project No. 3060-21000-039-00D, and the USDA-AMS Specialty Crop Block Grant Program 14-SCBGP-ND-0038. Mention of trade names or commercial products in this report is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The USDA is an equal opportunity provider and employer.

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  1. Department of Agronomy, Inner Mongolia Agricultural University, Huhhot, 010019, Inner Mongolia, China

    Z. W. Zhang & J. Zhao

  2. Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108, USA

    G. J. Ma & S. G. Markell

  3. Northern Crop Science Laboratory, USDA-Agricultural Research Service, 1605 Albrecht Blvd. N, Fargo, ND, 58102-2765, USA

    L. L. Qi

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Zhang, Z.W., Ma, G.J., Zhao, J. et al. Discovery and introgression of the wild sunflower-derived novel downy mildew resistance gene Pl 19 in confection sunflower (Helianthus annuus L.). Theor Appl Genet 130, 29–39 (2017). https://doi.org/10.1007/s00122-016-2786-z

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