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Genetic mapping of QTL for maize weevil resistance in a RIL population of tropical maize

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Abstract

Key message

A tropical RIL maize population was subjected to phenotypic and genotypic analysis for maize weevil resistance during four seasons, and three main genomic areas were detected as main QTLs.

Abstract

The maize weevil (Sitophilus zeamais) (MW) is a common and important pest of stored maize (Zea mays) worldwide, especially in tropical areas. Quantitative trait loci (QTLs) associated with the MW have been analyzed previously in an F2 maize population. In this work, new germplasm-based F6 recombinant inbred line (RIL) families, derived from the cross of Population 84 and Kilima, were analyzed using insect bioassays during four seasons. The parameters analyzed for MW resistance were grain weight losses (GWL), adult progeny (AP), and flour production (FP). Composite interval mapping identified a total of 15 QTLs for MW parameters located on six chromosomes, explaining between 14 and 51 % of phenotypic variation (σ 2p ) and 27 and 81 % of genotypic variation (σ 2g ). The QTL obtained for GWL was located in bin 2.05, which explained 15 % of σ 2p . For AP and FP, the QTLs were located on regions 1.09 and 2.05, explaining 7 and 15 % of σ 2p , respectively. Comparative analysis between F2 and F6 families showed similarities in QTL localization; three main regions were co-localized in chromosomes 4.08, 10.04, and 10.07, where no resistance-associated genes have been reported previously. These regions could be used for a marker-assisted selection in breeding programs for MW resistance in tropical maize.

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Abbreviations

GWL:

Grain weight losses

FP:

Flour production

AP:

Adult progeny

MW:

Maize weevil

QTL:

Quantitative trait locus

RIL:

Recombinant inbred line

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Acknowledgments

We gratefully acknowledge the support of the Consejo Nacional de Ciencia y Tecnologia, CONACYT-Mexico, by Research Funds of Basic Science (CB-2009-01-000000000134238) and CIMMYT, Research Funds.

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Plant-Food Molecular Breeding Unit, Tec de Monterrey, ITESM, C.P. 64849, Monterrey, NL, Mexico

    Fernando F. Castro-Álvarez & Silverio García-Lara

  2. Pioneer Hi-Bred International, Production LP, Woodstock, ON, Canada

    Manilal William

  3. Agricultural Development, Bill & Melinda Gates Foundation, PO Box 23350, Seattle, WA, 98102, USA

    David J. Bergvinson

Authors
  1. Fernando F. Castro-Álvarez
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  2. Manilal William
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  3. David J. Bergvinson
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  4. Silverio García-Lara
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Corresponding author

Correspondence to Silverio García-Lara.

Additional information

Communicated by Thomas Lubberstedt.

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Castro-Álvarez, F.F., William, M., Bergvinson, D.J. et al. Genetic mapping of QTL for maize weevil resistance in a RIL population of tropical maize. Theor Appl Genet 128, 411–419 (2015). https://doi.org/10.1007/s00122-014-2440-6

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  • DOI: https://doi.org/10.1007/s00122-014-2440-6

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