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QTL mapping for salt tolerance and domestication-related traits in Vigna marina subsp. oblonga, a halophytic species

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QTL mapping in F 2 population [ V. luteola × V. marina subsp. oblonga ] revealed that the salt tolerance in V. marina subsp. oblonga is controlled by a single major QTL.

Abstract

The habitats of beach cowpea (Vigna marina) are sandy beaches in tropical and subtropical regions. As a species that grows closest to the sea, it has potential to be a gene source for breeding salt-tolerant crops. We reported here for the first time, quantitative trait loci (QTLs) mapping for salt tolerance in V. marina. A genetic linkage map was constructed from an F2 population of 120 plants derived from an interspecific cross between V. luteola and V. marina subsp. oblonga. The map comprised 150 SSR markers. The markers were clustered into 11 linkage groups spanning 777.6 cM in length with a mean distance between the adjacent markers of 5.59 cM. The F2:3 population was evaluated for salt tolerance under hydroponic conditions at the seedling and developmental stages. Segregation analysis indicated that salt tolerance in V. marina is controlled by a few genes. Multiple interval mapping consistently identified one major QTL which can explain about 50 % of phenotypic variance. The flanking markers may facilitate transfer of the salt tolerance allele from V. marina subsp. oblonga into related Vigna crops. The QTL for domestication-related traits from V. marina are also discussed.

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Acknowledgments

This study was supported by JST, PRESTO to K. Naito, and by the Royal Golden Jubilee (RGJ) Ph.D. Program jointly funded by the Thailand Research Fund (TRF) and Kasetsart University to P. Srinives and S. Chankaew.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

All the experiments carried out in this study comply with the current laws of both Thailand and Japan.

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Authors and Affiliations

  1. Program in Plant Breeding, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen, Nakhon Pathom, 73140, Thailand

    Sompong Chankaew

  2. Genetic Resources Center, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan

    Takehisa Isemura, Ken Naito, Eri Ogiso-Tanaka, Norihiko Tomooka, Akito Kaga & Duncan A. Vaughan

  3. JST, Precursory Research for Embryonic Science and Technology (PRESTO), 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan

    Ken Naito

  4. Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen, Nakhon Pathom, 73140, Thailand

    Prakit Somta & Peerasak Srinives

Authors
  1. Sompong Chankaew
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  2. Takehisa Isemura
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  3. Ken Naito
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  4. Eri Ogiso-Tanaka
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  5. Norihiko Tomooka
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  6. Prakit Somta
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  7. Akito Kaga
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  8. Duncan A. Vaughan
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  9. Peerasak Srinives
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Corresponding authors

Correspondence to Ken Naito or Peerasak Srinives.

Additional information

Communicated by A. H. Schulman.

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Supplementary Figure S1. Leave wilt scores using the scales of 1 to 9 for salt tolerance evaluation in the F2:3 population (V. luteola x V. marina subsp. oblonga) after being maintained at 300 mM for 30 days.

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Supplementary material 2 (EPS 1886 kb)

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Chankaew, S., Isemura, T., Naito, K. et al. QTL mapping for salt tolerance and domestication-related traits in Vigna marina subsp. oblonga, a halophytic species. Theor Appl Genet 127, 691–702 (2014). https://doi.org/10.1007/s00122-013-2251-1

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