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Inheritance and QTL mapping of Fusarium wilt race 4 resistance in cotton

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Abstract

Diseases such as Fusarium wilt [Fusarium oxysporum f.sp. vasinfectum (FOV) Atk. Sny & Hans] represent expanding threats to cotton production. Integrating disease resistance into high-yielding, high-fiber quality cotton (Gossypium spp.) cultivars is one of the most important objectives in cotton breeding programs worldwide. In this study, we conducted a comprehensive analysis of gene action in cotton governing FOV race 4 resistance by combining conventional inheritance and quantitative trait loci (QTL) mapping with molecular markers. A set of diverse cotton populations was generated from crosses encompassing multiple genetic backgrounds. FOV race 4 resistance was investigated using seven parents and their derived populations: three intraspecific (G. hirsutum × G. hirsutum L. and G. barbadense × G. barbadense L.) F1 and F2; five interspecific (G. hirsutum × G. barbadense) F1 and F2; and one RIL. Parents and populations were evaluated for disease severity index (DSI) of leaves, and vascular stem and root staining (VRS) in four greenhouse and two field experiments. Initially, a single resistance gene (Fov4) model was observed in F2 populations based on inheritance of phenotypes. This single Fov4 gene had a major dominant gene action and conferred resistance to FOV race 4 in Pima-S6. The Fov4 gene appears to be located near a genome region on chromosome 14 marked with a QTL Fov4-C14 1 , which made the biggest contribution to the FOV race 4 resistance of the generated F2 progeny. Additional genetic and QTL analyses also identified a set of 11 SSR markers that indicated the involvement of more than one gene and gene interactions across six linkage groups/chromosomes (3, 6, 8, 14, 17, and 25) in the inheritance of FOV race 4 resistance. QTLs detected with minor effects in these populations explained 5–19 % of the DSI or VRS variation. Identified SSR markers for the resistance QTLs with major and minor effects will facilitate for the first time marker-assisted selection for the introgression of FOV race 4 resistance into elite cultivars during the breeding process.

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Acknowledgments

This study was funded in part by a Reimbursable Agreement with Cotton Incorporated (CA State Support Committee), Cary, NC (ARIS Log Nos. 5303-05-00 0029833), a Cooperative Research Agreement with Cotton Incorporated, and a University of California Discovery Grant. The authors thank Drs. R. Kohel and J. Yu for providing the original seed source of the RIL population. The authors also thank S. Ellberg, M. Biggs, Z. Garcia, M. Keeley, R. Delgado, G. Banuelos, and many students for their help in evaluations. Use of greenhouse facilities of the University of California Kearney Research and Extension Center (Parlier, CA) and assistance of Fred Swanson and Laura Van der Staay is gratefully acknowledged. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture or University of California. The US Department of Agriculture and University of California are equal opportunity providers and employers.

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Authors and Affiliations

  1. Cropping Systems Research Laboratory, Plant Stress and Germplasm Development Research, USDA-ARS, SPA, 3810 4th Street, Lubbock, TX, 79415, USA

    Mauricio Ulloa

  2. Department of Plant Sciences, University of California-Davis, Five Points, CA, 93624, USA

    Robert B. Hutmacher

  3. Department of Nematology, University of California, Riverside, CA, 92521, USA

    Philip A. Roberts

  4. University of California, Cooperative Extension, Tulare, CA, 93274, USA

    Steven D. Wright

  5. Cotton Incorporated, Cary, NC, 27513, USA

    Robert L. Nichols

  6. Department of Plant Pathology, University of California-Davis, Davis, CA, 95616, USA

    R. Michael Davis

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  1. Mauricio Ulloa
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  2. Robert B. Hutmacher
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  4. Steven D. Wright
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Correspondence to Mauricio Ulloa.

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Communicated by Y. Xue.

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Ulloa, M., Hutmacher, R.B., Roberts, P.A. et al. Inheritance and QTL mapping of Fusarium wilt race 4 resistance in cotton. Theor Appl Genet 126, 1405–1418 (2013). https://doi.org/10.1007/s00122-013-2061-5

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