Abstract
Facultative wheat varieties adapt to a particular environment. But the molecular basis for the facultative growth habit is not clear relative to winter and spring growth habit. Two sets of wheat varieties were chosen for this study. Set 1 comprised ten spring accessions and Set 2 comprised ten facultative accessions. All accessions had been tested by the previously described allele-specific markers and shown having the same allelic composition of vrn-A1 vrn-B1 Vrn-D1 and vrn-B3. Here we examined whether differences in growth habit might be associated with as yet unidentified sequence variation at Vrn-D1 locus. A region including the intron 1 deletion, the entire reading frame from a cDNA template and a part of promoter region of the dominant Vrn-D1 gene in each of the accessions was sequenced, and a single nucleotide polymorphism was found between facultative accessions and spring accessions in the CArG-box at the promoter region. The novel allele in facultative accessions was designated as Vrn-D1b. The investigation of an F2 population segregating for Vrn-D1b and Vrn-D1a (previously, Vrn-D1) in the greenhouse under long days without vernalization showed that the plants with Vrn-D1b homozygous allele headed 32 days later and had about three more leaves than the plants with Vrn-D1a homozygous allele. As Vrn-D1b has the same deletion in intron 1 as Vrn-D1a, and, in addition, a single nucleotide mutation at promoter region, and is associated with facultative growth habit, we suggest that the promoter mutation may modify the basal activity level of an allele of VRN1 that is already active (due to the loss of segments in intron 1). Our finding further supports that both the promoter and intron 1 regulatory affect vernalization response and work independently.
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References
Alonso-Peral MM, Oliver SN, Casao MC, Greenup AA, Trevaskis B (2011) The Promoter of the cereal vernalization1 gene is sufficient for transcriptional induction by prolonged cold. PLoS ONE 6(12):e29456
Fu D, Szücs P, Yan L, Helguera M, Skinner JS, Zitzewitz JV, Hayes PM, Dubcovsky J (2005) Large deletions within the first intron in VRN-1 are associated with spring growth habit in barley and wheat. Mol Genet Genomics 273:54–65
Goncharov NP (2003) Genetics of growth habit (spring vs winter) in common wheat: confirmation of the existence of dominant Vrn4. Theor Appl Genet 107:768–772
Iwaki K, Nishida J, Yanagisawa T, Yoshida H, Kato K (2002) Genetic analysis of Vrn-B1 for vernalization requirement by using linked dCAPS markers in bread wheat (Triticum aestivum L.). Theor Appl Genet 104:571–576
Jiang Y, Huang L, Hu Y (2010) Distribution of vernalization genes in Chinese wheat landraces and their relationship with winter hardness. Scientia Agricultura Sinica 43(13):2619–2632
Jin SB (1983) Chinese wheat varieties and their pedigrees. China Agricultural Publishing House, Beijing
Jin SB (1992) The theory and practice of wheat ecology. Zhejiang Publishing House Science and Technology, Hangzhou
Kane NA, Agharbaoui Z, Diallo AO, Adam H, Tominaga Y, Ouellet F, Sarhan F (2007) TaVRT2 represses transcription of the wheat vernalization gene TaVRN1. Plant J 51:670–680
Law CN, Worland AJ, Giorgi B (1976) The genetic control of ear-emergence time by chromosomes 5A and 5D of wheat. Heredity 36:49–58
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C (T)) method. Methods 25:402–408
Loukoianov A, Yan L, Blechl A, Sanchez A, Dubcovsky J (2005) Regulation of VRN-1 vernalization genes in normal and transgenic polyploid wheat. Plant Physiol 138:2364–2373
McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers J, Morris C, Somers DJ, Appels R, Devos KM (2008) Catalogue of gene symbols for wheat. In Proc. 11th Int. Wheat Genet. Symp., Brisbane, Queensland, 24–29 August 2008
Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325
Pugsley AT (1971) A genetic analysis of the spring-winter habit of growth in wheat. Aust J Agric Res 22:21–31
Pugsley AT (1972) Additional genes inhabiting winter habit in wheat. Euphytica 21:547–552
Snape JW, Butterworth K, Whitechurch E, Worland AJ (2001) Waiting for fine times: genetics of flowering time in wheat. Euphytica 119:185–190
Sun QM, Zhou RH, Gao LF, Zhao GY, Jia JZ (2009) The characterization and geographical distribution of the genes responsible for vernalization requirement in Chinese bread wheat. J Integr Plant Biol 51:423–432
Trevaskis B, Bagnall DJ, Ellis MH, Peacock WJ, Dennis ES (2003) MADS box genes control vernalization-induced flowering in cereals. Proc Natl Acad Sci USA 100:13099–13104
Trevaskis B, Hemming MN, Peacock WJ, Dennis ES (2006) HvVRN2 responds to daylength, whereas HvVRN1 is regulated by vernalization and developmental status. Plant Physiol 140:1397–1405
Wilson ID, Barker GLA, Beswick RW, Shepherd SK, Lu C, Coghill JA, Edwards D, Owen P, Lyon R, Parker JS, Lenton JR, Holdsworth MJ, Shewry PR, Edwards KJ (2004) A transcriptomics resource for wheat functional genomics. Plant Biotechnol J 2:495–506
Worland AJ (1996) The influence of flowering time genes on environmental adaptability in European wheats. Euphytica 89:49–57
Yan L, Loukoianov A, Tranquilli G, Helguera M, Fahima T, Dubcovsky J (2003) Positional cloning of wheat vernalization gene VRN1. Proc Natl Acad Sci USA 100:6263–6268
Yan L, Helguera M, Kato K, Fukuyama S, Sherman J, Dubcovsky J (2004) Allelic variation at the VRN-1 promoter region in polyploidy wheat. Theor Appl Genet 109:1677–1686
Yan L, Fu D, Li C, Blechl A, Tranquilli G, Bonafede M, Sanchez A, Valarik M, Yasuda S, Dubcovsky J (2006) The wheat and barley vernalization gene VRN3 is an orthologue of FT. Proc Natl Acad Sci USA 103:19581–19586
Yoshida T, Nishida H, Zhu J, Nitcher R, Distelfeld A, Akashi Y, Kato K, Dubcovsky J (2010) Vrn-D4 is a vernalization gene located on the centromeric region of chromosome 5D in hexaploid wheat. Theor Appl Genet 120:543–552
Zhang X, Xiao Y, Zhang Y, Xia X, Dubcovsky J, He Z (2008) Allelic variation at the vernalization genes Vrn-A1, Vrn-B1, Vrn-D1, and Vrn-B3 in Chinese wheat cultivars and their association with growth habit. Crop Sci 48:458–470
Zhang J, Wu SW, Liu BH, Song MF, Zhou P, Guo CY, Zhan KH, Wang SH, Yang L, Dong D, Yu LQ, Li HL, Yang JP, Zhou Y (2010) Genetic improvement of wheat growth habit and its molecular marker-assisted selection in Yellow and Huai river reaches. Acta Agronomica Sinica 36(3):290–385
Zhuang QS (2003) Wheat improvement and pedigree analysis in China. China Agricultural Publishing House, Beijing
Acknowledgments
We thank Dr Xianchun Xia and Dr Hongjie Li for their kindly reviewing this manuscript. This work was supported by the Ministry of Science and Technology of China (2009CB118301 and 2011BAD35B03) and the Ministry of Agriculture of China (2009ZX08002-006B).
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Communicated by J. Xiao.
J. Zhang and Y. Wang contributed equally to this work.
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Zhang, J., Wang, Y., Wu, S. et al. A single nucleotide polymorphism at the Vrn-D1 promoter region in common wheat is associated with vernalization response. Theor Appl Genet 125, 1697–1704 (2012). https://doi.org/10.1007/s00122-012-1946-z
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DOI: https://doi.org/10.1007/s00122-012-1946-z