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SSR-based genetic maps of Miscanthus sinensis and M. sacchariflorus, and their comparison to sorghum

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Abstract

We present SSR-based genetic maps from a cross between Miscanthus sacchariflorus Robustus and M. sinensis, the progenitors of the promising cellulosic biofuel feedstock Miscanthus × giganteus. cDNA-derived SSR markers were mapped by the two-way pseudo-testcross model due to the high heterozygosity of each parental species. A total of 261 loci were mapped in M. sacchariflorus, spanning 40 linkage groups and 1,998.8 cM, covering an estimated 72.7% of the genome. For M. sinensis, a total of 303 loci were mapped, forming 23 linkage groups and 2,238.3 cM, covering 84.9% of the genome. The use of cDNA-derived SSR loci permitted alignment of the Miscanthus linkage groups to the sorghum chromosomes, revealing a whole genome duplication affecting the Miscanthus lineage after the divergence of subtribes Sorghinae and Saccharinae, as well as traces of the pan-cereal whole genome duplication. While the present maps provide for many early research needs in this emerging crop, additional markers are also needed to improve map density and to further characterize the structural changes of the Miscanthus genome since its divergence from sorghum and Saccharum.

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Acknowledgments

This work was supported by the US Department of Energy-US Department of Agriculture Plant Feedstock Program (project grant number: 112786), the Consortium for Plant Biotechnology Research (CPBR), and Mendel Biotechnology.

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Authors and Affiliations

  1. Plant Genome Mapping Laboratory, University of Georgia, 111 Riverbend Road, Rm 228, Athens, GA, 30602, USA

    Changsoo Kim, Dong Zhang, Susan A. Auckland, Lisa K. Rainville & Andrew H. Paterson

  2. Mendel Biotechnology, 3935 Point Eden Way, Hayward, CA, 94545, USA

    Katrin Jakob & Brent Kronmiller

  3. Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA

    Erik J. Sacks

  4. Tinplant, Magdeburger Strasse 33, 39164, Klein Wanzleben, Germany

    Martin Deuter

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  1. Changsoo Kim
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  2. Dong Zhang
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Correspondence to Andrew H. Paterson.

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Communicated by E. Guiderdoni.

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Kim, C., Zhang, D., Auckland, S.A. et al. SSR-based genetic maps of Miscanthus sinensis and M. sacchariflorus, and their comparison to sorghum. Theor Appl Genet 124, 1325–1338 (2012). https://doi.org/10.1007/s00122-012-1790-1

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