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Recombination suppression at the dominant Rhg1/Rfs2 locus underlying soybean resistance to the cyst nematode

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Abstract

Host resistance to “yellow dwarf” or “moonlight” disease cause by any population (Hg type) of Heterodera glycines I., the soybean cyst nematode (SCN), requires a functional allele at rhg1. The host resistance encoded appears to mimic an apoptotic response in the giant cells formed at the nematode feeding site about 24–48 h after nematode feeding commences. Little is known about how the host response to infection is mediated but a linked set of 3 genes has been identified within the rhg1 locus. This study aimed to identify the role of the genes within the locus that includes a receptor-like kinase (RLK), a laccase and an ion antiporter. Used were near isogeneic lines (NILs) that contrasted at their rhg1 alleles, gene-based markers, and a new Hg type 0 and new recombination events. A syntenic gene cluster on Lg B1 was found. The effectiveness of SNP probes from the RLK for distinguishing homolog sequence variants on LgB1 from alleles at the rhg1 locus on LgG was shown. The resistant allele of the rhg1 locus was shown to be dominant in NILs. None of the recombination events were within the cluster of the three candidate genes. Finally, rhg1 was shown to reduce the plant root development. A model for rhg1 as a dominant multi-gene resistance locus based on the developmental control was inferred.

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Acknowledgments

The physical map was based upon work supported by the National Science Foundation under Grant No. 9872635. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation. The continued support of SIUC, College of Agriculture and Office of the Vice Chancellor for Research to DAL is appreciated. The authors thank Dr. Q. Tao and Dr, H.B. Zhang for assistance with fingerprinting. We thank Dr. C. Town and Dr. C. Foo at TIGR for the BES. We thank the DOE CJSP for release of the WGS reads and scaffolds. We thank the Government of India for support of the Fellowship for NS.

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  1. Ahmed J. Afzal

    Present address: Department of Horticulture and Crop Science, Ohio State University, 2021 Coffey Rd, Columbus, OH, 43210, USA

  2. Navinder Saini

    Present address: Department of Biotechnology and Molecular Biology, CCS Haryana Agricultural University, Hisar, India

  3. Hany A. El Shemy

    Present address: Biotechnology Labs, FARP, Biochemistry and Molecular Biology, Biochemistry Department, Faculty of Agriculture, Cairo University, 12613, Giza, Egypt

Authors and Affiliations

  1. Department of Molecular Biology, Microbiology and Biochemistry, Southern Illinois University at Carbondale, Carbondale, IL, 62901, USA

    Ahmed J. Afzal, Ali Srour & David A. Lightfoot

  2. Genomics Core Facility, Department of Plant Soil and Agricultural Systems, Center for Excellence in Soybean Research, Teaching and Outreach, Southern Illinois University at Carbondale, Carbondale, IL, 62901-4415, USA

    Ahmed J. Afzal, Ali Srour, Navinder Saini, Naghmeh Hemmati, Hany A. El Shemy & David A. Lightfoot

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  1. Ahmed J. Afzal
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Correspondence to David A. Lightfoot.

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Communicated by I. Rajcan.

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Afzal, A.J., Srour, A., Saini, N. et al. Recombination suppression at the dominant Rhg1/Rfs2 locus underlying soybean resistance to the cyst nematode. Theor Appl Genet 124, 1027–1039 (2012). https://doi.org/10.1007/s00122-011-1766-6

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