Skip to main content
SpringerLink
Log in

Fine-mapping of qRfg2, a QTL for resistance to Gibberella stalk rot in maize

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Stalk rot is one of the most devastating diseases in maize worldwide. In our previous study, two QTLs, a major qRfg1 and a minor qRfg2, were identified in the resistant inbred line ‘1145’ to confer resistance to Gibberella stalk rot. In the present study, we report on fine-mapping of the minor qRfg2 that is located on chromosome 1 and account for ~8.9% of the total phenotypic variation. A total of 22 markers were developed in the qRfg2 region to resolve recombinants. The progeny-test mapping strategy was developed to accurately determine the phenotypes of all recombinants for fine-mapping of the qRfg2 locus. This fine-mapping process was performed from BC4F1 to BC8F1 generations to narrow down the qRfg2 locus into ~300 kb, flanked by the markers SSRZ319 and CAPSZ459. A predicted gene in the mapped region, coding for an auxin-regulated protein, is believed to be a candidate for qRfg2. The qRfg2 locus could steadily increase the resistance percentage by ~12% across different backcross generations, suggesting its usefulness in enhancing maize resistance against Gibberella stalk rot.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Abad L, Wolters P, Stucker D, Davis P (2006) Advances in anthracnose stalk rot resistance. The fifth national IPM symposium, “delivering on a promise”. http://www.ipmcenters.org/ipmsymposiumv/posters/037.pdf

  • Ahmad Y, Hameed A, Aslam M (1996) Effect of soil solarization on corn stalk rot. Plant Soil 179:17–24

    Article  CAS  Google Scholar 

  • Andrew RH (1954) Breeding for stalk-rot resistance in maize. Euphytica 3:43–45

    Article  Google Scholar 

  • Basten CJ, Weir BS, Zeng ZB (1997) QTL cartographer: a reference manual and tutorial for QTL mapping. Department of Statistics, North Carolina State University, Raleigh

  • Chen J (2000) Status and perspective on research of ear rot and stalk rot in maize. J Shenyang Agric Univ 31:393–401

    Google Scholar 

  • Chen SJ, Song TM (1999) Disease resistance of maize stalk rot. Simple genetics controlled by a single gene. Acta China Agric Univ 4:56

    Google Scholar 

  • Chen Z, Agnew JL, Cohen JD, He P, Shan L, Sheen J, Kunkel BN (2007) Pseudomonas syringae type III effector AvrRpt2 alters Arabidopsis thaliana auxin physiology. Proc Natl Acad Sci USA 104:20131–20136

    Article  PubMed  CAS  Google Scholar 

  • Christensen JJ, Wilcoxson RD (1966) Stalk rot of corn. Am Phytopathol Soc Monograph 3:1–59

    Google Scholar 

  • Chung KR, Shilts T, Erturk U, Timmer LW, Ueng PP (2003) Indole derivatives produced by the fungus Colletotrichum acutatum causing lime anthracnose and postbloom fruit drop of citrus. FEMS Microbiol Lett 226:23–30

    Article  PubMed  CAS  Google Scholar 

  • Clark RM, Wagler TN, Quijada P, Doebley J (2006) A distant upstream enhancer at the maize domestication gene tb1 has pleiotropic effects on plant and inflorescent architecture. Nat Genet 38:594–597

    Article  PubMed  CAS  Google Scholar 

  • Colbert TR, Kang MS, Myers O, Zuber MS (1987) General and specific combining ability estimates for pith cell death in stalk internodes of maize. Field Crops Res 17:155–162

    Article  Google Scholar 

  • Collard BCY, Jahufer MZZ, Brouwer JB, Pang ECK (2005) An introduction to markers, quantitative trait loci (QTL) mapping and marker-assisted selection for crop improvement: the basic concepts. Euphytica 142:169–196

    Article  CAS  Google Scholar 

  • Daetwyler HD, Pong-Wong R, Villanueva B, Woolliams JA (2010) The impact of genetic architecture on genome-wide evaluation methods. Genetics 185:1021–1031

    Article  PubMed  CAS  Google Scholar 

  • Ding XH, Cao YL, Huang LL, Zhao J, Xu CG, Li XH, Wang SP (2008) Activation of the indole-3-acetic acid-amido synthetase GH3–8 suppresses expansin expression and promotes salicylate- and jasmonate-independent basal immunity in rice. Plant Cell 20:228–240

    Article  PubMed  CAS  Google Scholar 

  • Doerge RW, Zeng ZB, Weir BS (1997) Statistical issues in the search for genes affecting quantitative traits in experimental populations. Stat Sci 12:195–219

    Article  Google Scholar 

  • Dorn B, Forrer HR, Schürch S, Vogelgsang S (2009) Fusarium species complex on maize in Switzerland: occurrence, prevalence, impact and mycotoxins in commercial hybrids under natural infection. Eur J Plant Pathol 125:51–61

    Article  CAS  Google Scholar 

  • Ducrocq S, Giauffret C, Madur D, Combes V, Dumas F, Jouanne S, Coubriche D, Jamin P, Moreau L, Charcosset A (2009) Fine-mapping and haplotype structure analysis of a major flowering time quantitative trait locus on maize chromosome 10. Genetics 183:1555–1563

    Article  PubMed  CAS  Google Scholar 

  • Flint J, Mackay TFC (2009) Genetic architecture of quantitative traits in flies, mice and humans. Genome Res 19:723–733

    Article  PubMed  CAS  Google Scholar 

  • Fu DL, Uauy C, Distelfeld A, Blechl A, Epstein L, Chen XM, Sela H, Fahima T, Dubcovsky J (2009) A kinase-START gene confers temperature-dependent resistance to wheat stripe rust. Science 323:1357–1360

    Article  PubMed  CAS  Google Scholar 

  • Holland JB (2007) Genetic architecture of complex traits in plants. Curr Opin Plant Biol 10:156–161

    Article  PubMed  CAS  Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Article  Google Scholar 

  • Kou YJ, Wang SP (2010) Broad-spectrum and durability: understanding of quantitative disease resistance. Curr Opin Plant Biol 13:1–5

    Article  Google Scholar 

  • Krattinger SG, Lagudah ES, Spielmeyer W, Singh RP, Huerta-Espino J, McFadden H, Bossolini E, Selter LL, Keller B (2009) A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science 323:1360–1362

    Article  PubMed  CAS  Google Scholar 

  • Lal S, Singh IS (1984) Breeding for resistance to downy mildews and stalk rots in maize. Theor Appl Genet 69:111–119

    Google Scholar 

  • Li Q, Wan JM (2005) SSRHunter: development of a local searching software for SSR sites. Hereditas (Beijing) 27:808–810

    Google Scholar 

  • Lincoln S, Daly M, Lander E (1992) Mapping genes controlling quantitative traits with MAPMAKER/QTL 1.1. Whitehead Institute Technical Report, 2nd edn

  • Mackay TFC, Stone EA, Ayroles JF (2009) The genetics of quantitative traits: challenges and prospects. Nat Rev Genet 10:565–577

    Article  PubMed  CAS  Google Scholar 

  • Manulis S, Shafrir H, Epstein E, Lichter A, Barash I (1994) Biosynthesis of indole-3-acetic acid via the indole-3-acetamide pathway in Streptomyces spp. Microbiology 140:1045–1050

    Article  PubMed  CAS  Google Scholar 

  • Maor R, Haskin S, Levi-Kedmi H, Sharon A (2004) In planta production of indole-3-acetic acid by Colletotrichum gloeosporioides f. sp. aeschynomene. Appl Environ Microbiol 70:1852–1854

    Article  PubMed  CAS  Google Scholar 

  • Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4326

    Article  PubMed  CAS  Google Scholar 

  • Navarro L, Dunoyer P, Jay F, Arnold B, Dharmasiri N, Estelle M, Voinnet O, Jones JDG (2006) A plant miRNA contributes to antibacterial resistance by repressing auxin signaling. Science 312:436–439

    Article  PubMed  CAS  Google Scholar 

  • Nobuta K, Okrent RA, Stoutemyer M, Rodibaugh N, Kempema L, Wildermuth MC, Innes RW (2007) The GH3 acyl adenylase family member PBS3 regulates salicylic acid-dependent defense responses in Arabidopsis. Plant Physiol 144:1144–1156

    Article  PubMed  CAS  Google Scholar 

  • Park JE, Park JY, Kim YS, Staswick PE, Jeon J, Yun J, Kim SY, Kim J, Lee YH, Park CM (2007) GH3-mediated auxin homeostasis links growth regulation with stress adaptation response in Arabidopsis. J Biol Chem 282:10036–10046

    Article  PubMed  CAS  Google Scholar 

  • Pè ME, Gianfranceschi L, Taramino G, Tarchini R, Angelini P, Dani M, Binelli G (1993) Mapping quantitative trait loci (QTLs) for resistance to Gibberella zeae infection in maize. Mol Gen Genet 241:11–16

    Article  PubMed  Google Scholar 

  • Russel WA (1961) A comparison of five types of testers in evaluating the relationship of stalk rot resistance in com inbred fines and stalk strength of the lines in hybrid combinations. Crop Sci 1:393–397

    Article  Google Scholar 

  • Sax K (1923) The association of size differences with seed coat pattern and pigmentation in Phaseolus vulgaris. Genetics 8:522–560

    Google Scholar 

  • Saxena SC (1982) Epidemiology and control of Erwinia stalk rot of maize. PhD Thesis, GB Pant Univ Agric Techn, Pantnagar, India

  • Sharma RC, Leon CD, Payak MM (1993) Diseases of maize in South and South-East Asia: problems and progress. Crop Prot 12:414–422

    Article  Google Scholar 

  • Shim WB, Sagaram US, Choi YE, So J, Wilkinson HH, Lee YW (2006) FSR1 is essential for virulence and female fertility in Fusarium verticillioides and F. graminearum. MPMI 19:725–733

    Article  PubMed  CAS  Google Scholar 

  • Song XJ, Huang W, Shi M, Zhu MZ, Lin HX (2007) A QTL for rice grain width and weight encodes a previously unknown RING-type E3 ubiquitin ligase. Nat Genet 39:623–630

    Article  PubMed  CAS  Google Scholar 

  • Szőke C, Árendás T, Rácz F, Pintér J, Nagy E, Marton CL (2007) Correlation between maize genotypes and the stalk rot caused by maize Fusarium. Acta Agron Hung 55:447–452

    Article  Google Scholar 

  • Vandeputte O, Oden S, Mol A, Vereecke D, Goethals K, El Jaziri M, Prinsen E (2005) Biosynthesis of auxin by the gram-positive phytopathogen Rhodococcus fascians is controlled by compounds specific to infected plant tissues. Appl Environ Microbiol 71:1169–1177

    Article  PubMed  CAS  Google Scholar 

  • Wang H, Nussbaum-Wagler T, Li BL, Zhao Q, Vigouroux Y, Faller M, Bomblies K, Lukens L, Doebley JF (2005) The origin of the naked grains of maize. Nature 436:714–719

    Article  PubMed  CAS  Google Scholar 

  • Wang D, Pajerowska-Mukhtar K, Culler AH, Dong X (2007) Salicylic acid inhibits pathogen growth in plants through repression of the auxin signaling pathway. Curr Biol 17:1784–1790

    Article  PubMed  CAS  Google Scholar 

  • White DG (1999) Compendium of corn diseases, 3rd edn. APS Press, Saint Paul, p 78

    Google Scholar 

  • Widakas W, Sandaland PC, Liu H (1980) Inheritance of resistance to Diplodia zeae, Gibberella zeae and Fusarium moniliforme stalk rot in corn. Maize Genet Coop Newsl 34:97–98

    Google Scholar 

  • Wu HY, Sun SR, Fan ZW, Liu CG, Yang TY, Zhu JH (2007) Research Condition and Prevention Countermeas ures of Maize Stalk Rot. J Maize Sci 15:129–132

    Google Scholar 

  • Yang DE, Zhang CL, Zhang DS, Jin DM, Weng ML, Chen SJ, Nguyen H, Wang B (2004) Genetic analysis and molecular mapping of maize (Zea mays L.) stalk rot resistant gene Rfg1. Theor Appl Genet 108:706–711

    Article  PubMed  CAS  Google Scholar 

  • Yang Q, Yin GM, Guo YL, Zhang DF, Chen SJ, Xu ML (2010) A major QTL for resistance to Gibberella stalk rot in maize. Theor Appl Genet 121:673–687

    Article  PubMed  Google Scholar 

  • Younis SEA, Abo-El Dahab MK, Mallah GS (1969) Genetic studies of the resistance to Fusarium stalk rot in maize. Indian J Genet 29:418–425

    Google Scholar 

  • Zhang Z, Li Q, Li Z, Staswick PE, Wang M, Zhu Y, He Z (2007) Dual regulation role of GH3.5 in salicylic acid and auxin signaling during Arabidopsis–Pseudomonas syringae interaction. Plant Physiol 145:450–464

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors cordially thank Mr. Chao Wang and Ms. Nan Zhang for their technical assistance in both genotyping and field evaluation. This study was financially supported by the National ‘863’ High-Tech Program of China and the National ‘973’ Basic Research Program, Grant No: 2009CB118401.

Author information

Authors and Affiliations

  1. National Maize Improvement Center of China, China Agricultural University, 2 West Yuanmingyuan Road, Beijing, 100193, People’s Republic of China

    Dongfeng Zhang, Yongjie Liu, Yanling Guo, Qin Yang, Jianrong Ye, Shaojiang Chen & Mingliang Xu

Authors
  1. Dongfeng Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yongjie Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yanling Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Qin Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jianrong Ye
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Shaojiang Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Mingliang Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mingliang Xu.

Additional information

Communicated by T. Luebberstedt.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, D., Liu, Y., Guo, Y. et al. Fine-mapping of qRfg2, a QTL for resistance to Gibberella stalk rot in maize. Theor Appl Genet 124, 585–596 (2012). https://doi.org/10.1007/s00122-011-1731-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-011-1731-4

Keywords

Search

Navigation