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Quantitative trait loci and underlying candidate genes controlling agronomical and fruit quality traits in octoploid strawberry (Fragaria × ananassa)

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Abstract

Breeding for fruit quality traits in strawberry (Fragaria × ananassa, 2n = 8x = 56) is complex due to the polygenic nature of these traits and the octoploid constitution of this species. In order to improve the efficiency of genotype selection, the identification of quantitative trait loci (QTL) and associated molecular markers will constitute a valuable tool for breeding programs. However, the implementation of these markers in breeding programs depends upon the complexity and stability of QTLs across different environments. In this work, the genetic control of 17 agronomical and fruit quality traits was investigated in strawberry using a F1 population derived from an intraspecific cross between two contrasting selection lines, ‘232’ and ‘1392’. QTL analyses were performed over three successive years based on the separate parental linkage maps and a pseudo-testcross strategy. The integrated strawberry genetic map consists of 338 molecular markers covering 37 linkage groups, thus exceeding the 28 chromosomes. 33 QTLs were identified for 14 of the 17 studied traits and approximately 37% of them were stable over time. For each trait, 1–5 QTLs were identified with individual effects ranging between 9.2 and 30.5% of the phenotypic variation, indicating that all analysed traits are complex and quantitatively inherited. Many QTLs controlling correlated traits were co-located in homoeology group V, indicating linkage or pleiotropic effects of loci. Candidate genes for several QTLs controlling yield, anthocyanins, firmness and l-ascorbic acid are proposed based on both their co-localization and predicted function. We also report conserved QTLs among strawberry and other Rosaceae based on their syntenic location.

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Acknowledgments

This work was supported by grants RTA2008-00029-00-00 (INIA, partly funded by FEDER, European Union), AGR-03230 (CICE, Junta de Andalucía) and BIO2010-15630 (MEC). Y.Z-F. and I.A. were supported by a fellowship and a contract, respectively, from INIA. The authors would like to thank Luis Miranda for his excellent technical assistance in the field, Dr. E. Martínez-Ferri for statistical support, Dr. F. Csukasi for kindly providing the genomic sequence of FaTIR1, Dr. J.W. van Ooijen for helpful advice on mapping and QTL analysis and Dr. M.A. Botella for stimulating discussions and critical reading of the manuscript.

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Authors and Affiliations

  1. IFAPA-Centro de Churriana, Cortijo de la Cruz S/N, Churriana, 29140, Málaga, Spain

    Yasmín Zorrilla-Fontanesi, Amalia Cabeza, José F. Sánchez-Sevilla & Iraida Amaya

  2. IFAPA-Centro Las Torres, Alcalá Del Río, Km 12.2, Sevilla, Spain

    Pedro Domínguez & Juan Jesús Medina

  3. Departamento de Biología Molecular y Bioquímica, Instituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), 29071, Málaga, Spain

    Victoriano Valpuesta

  4. Institut National de la Recherche Agronomique, UREF, BP81, INRA, UR 419, 33883, Villenave d’Ornon Cedex, France

    Beatrice Denoyes-Rothan

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  1. Yasmín Zorrilla-Fontanesi
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  2. Amalia Cabeza
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  7. José F. Sánchez-Sevilla
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Correspondence to Iraida Amaya.

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Communicated by H. Nybom.

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Zorrilla-Fontanesi, Y., Cabeza, A., Domínguez, P. et al. Quantitative trait loci and underlying candidate genes controlling agronomical and fruit quality traits in octoploid strawberry (Fragaria × ananassa). Theor Appl Genet 123, 755–778 (2011). https://doi.org/10.1007/s00122-011-1624-6

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