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Positional cloning of ds1, the target leaf spot resistance gene against Bipolaris sorghicola in sorghum

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Abstract

Target leaf spot is one of the major sorghum diseases in southern Japan and caused by a necrotrophic fungus, Bipolaris sorghicola. Sorghum resistance to target leaf spot is controlled by a single recessive gene (ds1). A high-density genetic map of the ds1 locus was constructed with simple sequence repeat markers using progeny from crosses between a sensitive variety, bmr-6, and a resistant one, SIL-05, which allowed the ds1 gene to be genetically located within a 26-kb region on the short arm of sorghum chromosome 5. The sorghum genome annotation database for BTx623, for which the whole genome sequence was recently published, indicated a candidate gene from the Leucine-Rich Repeat Receptor Kinase family in this region. The candidate protein kinase gene was expressed in susceptible plants but was not expressed or was severely reduced in resistant plants. The expression patterns of ds1 gene and the phenotype of target leaf spot resistance were clearly correlated. Genomic sequences of this region in parental varieties showed a deletion in the promoter region of SIL-05 that could cause reduction of gene expression. We also found two ds1 alleles for resistant phenotypes with a stop codon in the coding region. The results shown here strongly suggest that the loss of function or suppression of the ds1 protein kinase gene leads to resistance to target leaf spot in sorghum.

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Acknowledgments

We thank Dr. Takao Tsukiboshi for providing Bipolaris sorghicola and for his valuable experimental suggestions for dealing with the fungi. We also thank Mr. Futa Sakakibara and Ms. Teiko Tanaka for their support in the field work. This work was supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Genomics for Agricultural Innovation, SOR-0002)

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Authors and Affiliations

  1. National Institute of Agrobiological Sciences, Tsukuba, 305-8602, Japan

    Hiroyuki Kawahigashi, Jianzhong Wu, Jun-ichi Yonemaru & Takashi Matsumoto

  2. Faculty of Agriculture, Shinshu University, 8304, Minamiminowa, Nagano, 399-4598, Japan

    Shigemitsu Kasuga

  3. Institute of the Society for Techno-innovation of Agriculture, Forestry and Fisheries, 446-1 Ippaizuka, Kamiyokoba, Tsukuba, 305-0854, Japan

    Tsuyu Ando & Hiroyuki Kanamori

  4. Bioscience and Biotechnology Center, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601, Japan

    Takashi Sazuka

  5. Plant Genome Research Unit, National Institute of Agrobiological Sciences, 2-1-2, Kan-non-dai, Tsukuba, 305-8602, Japan

    Hiroyuki Kawahigashi

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  1. Hiroyuki Kawahigashi
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  2. Shigemitsu Kasuga
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Correspondence to Hiroyuki Kawahigashi.

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Communicated by A. Graner.

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Kawahigashi, H., Kasuga, S., Ando, T. et al. Positional cloning of ds1, the target leaf spot resistance gene against Bipolaris sorghicola in sorghum. Theor Appl Genet 123, 131–142 (2011). https://doi.org/10.1007/s00122-011-1572-1

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