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Genetic mapping of DArT markers in the Festuca–Lolium complex and their use in freezing tolerance association analysis

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Abstract

Species belonging to the Festuca–Lolium complex are important forage and turf species and as such, have been studied intensively. However, their out-crossing nature and limited availability of molecular markers make genetic studies difficult. Here, we report on saturation of F. pratensis and L. multiflorum genetic maps using Diversity Array Technology (DArT) markers and the DArTFest array.The 530 and 149 DArT markers were placed on genetic maps of L. multiflorum and F. pratensis, respectively, with overlap of 20 markers, which mapped in both species. The markers were sequenced and comparative sequence analysis was performed between L. multiflorum, rice and Brachypodium. The utility of the DArTFest array was then tested on a Festulolium population FuRs0357 in an integrated analysis using the DArT marker map positions to study associations between markers and freezing tolerance. Ninety six markers were significantly associated with freezing tolerance and five of these markers were genetically mapped to chromosomes 2, 4 and 7. Three genomic loci associated with freezing tolerance in the FuRs0357 population co-localized with chromosome segments and QTLs previously indentified to be associated with freezing tolerance. The present work clearly confirms the potential of the DArTFest array in genetic studies of the Festuca–Lolium complex. The annotated DArTFest array resources could accelerate further studies and improvement of desired traits in Festuca–Lolium species.

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References

  • Akbari M, Wenzl P, Caig V, Carling J, Xia L, Yang SY, Uszynski G, Mohler V, Lehmensiek A, Kuchel H, Hayden MJ, Howes N, Sharp P, Vaughan P, Rathmell B, Huttner E, Kilian A (2006) Diversity arrays technology (DArT) for high-throughput profiling of the hexaploid wheat genome. Theor Appl Genet 113:1409–1420

    Article  PubMed  CAS  Google Scholar 

  • Alm V, Fang C, Busso CS, Devos KM, Vollan K, Grieg Z, Rognli OA (2003) A linkage map of meadow fescue (Festuca pratensis Huds.). Theor Appl Genet 108:25–40

    Article  PubMed  CAS  Google Scholar 

  • Bennett MD, Leitch IJ (2005) Plant DNA C-values database (release 4.0, Oct. 2005). http://www.kew.org/cvalues/

  • Bolibok-Bragoszewska H, Heller-Uszyńska K, Wenzl P, Uszyński G, Kilian A, Rakoczy-Trojanowska M (2009) DArT markers for the rye genome—genetic diversity and mapping. BMC Genomics 10:578

    Article  PubMed  Google Scholar 

  • Bolot S, Abrouk M, Masood-Quraishi U, Stein N, Messing J, Feuillet C, Salse J (2009) The ‘inner circle’ of the cereal genomes. Curr Opin Plant Biol 12:119–125

    Article  PubMed  CAS  Google Scholar 

  • Catalán P, Torrecilla P, Rodriguez JÁL, Olmstead RG (2004) Phylogeny of the festucoid grasses of subtribe Loliinae and allies (Poeae, Pooideae) inferred from ITS and trnL-F sequences. Mol Phylogenet Evol 31:517–541

    Article  PubMed  Google Scholar 

  • Cattivelli L, Baldi P, Crosatti C, Di Fonzo N, Faccioli P, Grossi M, Mastrangelo AM, Pecchioni N, Stanca AM (2002) Chromosome regions and stress-related sequences involved in resistance to abiotic stress in Triticeae. Plant Mol Biol 48:649–665

    Article  CAS  Google Scholar 

  • Conesa A, Götz S, García-Gómez JM, Terol J, Talón M, Robles M (2005) Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics 21:3674–3676

    Article  PubMed  CAS  Google Scholar 

  • Dhillon T, Pearce SP, Stockinger EJ, Distelfeld A, Li C, Knox AK, Vashegyi I, Vágújfalvi A, Galiba G, Dubcovsky J (2010) Regulation of freezing tolerance and flowering in temperate cereals: the VRN-1 connection. Plant Physiol 153:1846–1858

    Article  PubMed  CAS  Google Scholar 

  • Ergon Å, Fang C, Jørgensen Ø, Aamlid TS, Rognli OA (2006) Quantitative trait loci controlling vernalisation requirement heading time and number of panicles in meadow fescue (Festuca pratensis Huds.). Theor Appl Genet 112:232–242

    Article  PubMed  CAS  Google Scholar 

  • Fjellheim S, Rognli OA, Fosnes K, Brochmann C (2006) Recent bottlenecking in the widespread meadow fescue (Festuca pratensis Huds.) inferred from chloroplast DNA sequences. J Biogeogr 33:1470–1478

    Article  Google Scholar 

  • Galiba G, Vágújfalvi A, Li C, Soltész A, Dubcovsky J (2009) Regulatory genes involved in the determination of frost tolerance in temperate cereals. Plant Sci 176:12–19

    Article  CAS  Google Scholar 

  • Hanada K, Zou C, Lehti-Shiu MD, Shinozaki K, Shiu SH (2008) Importance of lineage-specific expansion of plant tandem duplicates in the adaptive response to environmental stimuli. Plant Physiol 148:993–1003

    Article  PubMed  CAS  Google Scholar 

  • Higgins JA, Bailey PC, Laurie DA (2010) Comparative genomics of flowering time pathways using Brachypodium distachyon as a model for the temperate grasses. PLoS ONE 5:e10065

    Article  PubMed  Google Scholar 

  • Humphreys J, Harper JA, Armstead IP, Humphreys MW (2005) Introgression-mapping of genes for drought resistance transferred from Festuca arundinacea var. glaucescens into Lolium multiflorum. Theor Appl Genet 110:579–587

    Article  PubMed  CAS  Google Scholar 

  • Jaccoud D, Peng K, Feinstein D, Kilian A (2001) Diversity Arrays: a solid state technology for sequence information independent genotyping. Nucleic Acids Res 29:e25

    Article  PubMed  CAS  Google Scholar 

  • Jackson S, Chen J (2010) Genomic and expression plasticity of polyploidy. Curr Opin Plant Biol 13:153–159

    Article  PubMed  CAS  Google Scholar 

  • Kopecký D, Lukaszewski AJ, Doležel J (2005) Genomic constitution of Festulolium cultivars released in the Czech Republic. Plant Breeding 124:454–458

    Article  Google Scholar 

  • Kopecký D, Bartoš J, Lukaszewski AJ, Baird JH, Černoch V, Kölliker R, Rognli OA, Blois H, Caig V, Lübberstedt T, Studer B, Shaw P, Doležel J, Andrzej Kilian A (2009) Development and mapping of DArT markers within the Festuca–Lolium complex. BMC Genomics 10:473

    Article  PubMed  Google Scholar 

  • Kopecký D, Bartoš J, Christelová P, Černoch V, Kilian A, Doležel J (2011) Genomic constitution of Festuca × Lolium hybrids revealed by the DArTFest array. Theor Appl Genet. doi:10.1007/s00122-010-1451-1

  • Kosmala A, Zwierzykowski Z, Gasior D, Rapacz M, Zwierzykowska E, Humphreys MW (2006) GISH/FISH mapping of genes for freezing tolerance transferred from Festuca pratensis to Lolium multiflorum. Heredity 96:243–251

    Article  PubMed  CAS  Google Scholar 

  • Larsen A (1978) Freezing tolerance in grasses. Methods for testing in controlled environments. Meld Norg Landbr Høgsk 57:1–56

    Google Scholar 

  • Larsen A (1979) Freezing tolerance in grasses. Variation within populations and responce to selection. Meld Norg Landbr Høgsk 58:1–28

    Google Scholar 

  • Larsen A (1994) Breeding winter hardy grasses. Euphytica 77:231–237

    Article  Google Scholar 

  • Ouyang S, Buell CR (2004) The TIGR plant repeat databases: a collective resource for the identification of repetitive sequences in plants. Nucleic Acids Res 32(Database issue):D360–D363

    Google Scholar 

  • Peter-Schmid M, Boller B, Kölliker R (2008) Habitat and management affect genetic structure of Festuca pratensis but not Lolium multiflorum ecotype populations. Plant Breed 127:510–517

    Article  Google Scholar 

  • Preston JC, Kellogg EA (2007) Conservation and divergence of APETALA1/FRUITFULL-like gene function in grasses: evidence from gene expression analyses. Plant J 52:69–81

    Article  PubMed  CAS  Google Scholar 

  • R Development Core Team (2009) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0, URL http://www.R-project.org

  • Rizzon C, Ponger L, Gaut BS (2006) Striking similarities in the genomic distribution of tandemly arrayed genes in Arabidopsis and rice. PLoS Comput Biol 2:e115

    Article  PubMed  Google Scholar 

  • Rognli OA, Saha MC, Bhamidimarri S, van der Hejden S (2010) Fescues. In ‘B. Boller et al. (eds) Fodder cops and Amenity grasses, handbook of plant breeding, vol 5. doi:10.1007/978-1-4419-0760-8_11, Springer Science + Business Media, pp 261–292

  • Shinozuka H, Hisano H, Yoneyama S, Shimamoto Y, Jones ES, Forster JW, Yamada T, Kanazawa A (2006) Gene expression and genetic mapping analyses of a perennial ryegrass glycine-rich RNA-binding protein gene suggest a role in cold adaptation. Mol Genet Genomics 275:399–408

    Article  PubMed  CAS  Google Scholar 

  • Singh PK, Mergoum M, Adhikari TB, Shah T, Ghavami F, Kianian SF (2010) Genetic and molecular analysis of wheat tan spot resistance effective against Pyrenophora tritici-repentis races 2 and 5. Mol Breed 25:369–379

    Article  CAS  Google Scholar 

  • Storey JD (2002) A direct approach to false discovery rates. J R Stat Soc Ser B 64:479–498

    Article  Google Scholar 

  • Studer B, Boller B, Herrmann D, Bauer E, Posselt UK, Widmer F, Kölliker R (2006) Genetic mapping reveals a single major QTL for bacterial wilt resistance in Italian ryegrass (Lolium multiflorum Lam.). Theor Appl Genet 113:661–671

    Article  PubMed  CAS  Google Scholar 

  • Studer B, Boller B, Bauer E, Posselt UK, Widmer F, Kölliker R (2007) Consistent detection of QTLs for crown rust resistance in Italian ryegrass (Lolium multiflorum Lam.) across environments and phenotyping methods. Theor Appl Genet 115:9–17

    Article  PubMed  Google Scholar 

  • Studer B, Kölliker R, Muylle H, Asp T, Frei U, Roldán-Ruiz I, Barre P, Barth S, Skøt L, Armstead IP, Dolstra O, Roulund N, Nielsen KK, Lübberstedt T (2010) EST-derived SSR markers used as anchor loci for the construction of a consensus linkage map in ryegrass (Lolium spp.). BMC Plant Biol 10:177

    Article  PubMed  Google Scholar 

  • Sugiyama S (1998) Differentiation in competitive ability and cold tolerance between diploid and tetraploid cultivars in Lolium perenne. Euphytica 103:55–59

    Article  Google Scholar 

  • The International Brachypodium Initiative (2010) Genome sequencing and analysis of the model grass Brachypodium distachyon. Nature 463:763–768

    Article  Google Scholar 

  • Tinker NA, Kilian A, Wight CP, Heller-Uszynska K, Wenzl P, Rines HW, Bjørnstad A, Howarth CJ, Jannink JL, Anderson JM, Rossnagel BG, Stuthman DD, Sorrells ME, Jackson EW, Tuvesson S, Kolb FL, Olsson O, Federizzi LC, Carson ML, Ohm HW, Molnar SJ, Scoles GJ, Eckstein PE, Bonman JM, Ceplitis A, Langdon T (2009) New DArT markers for oat provide enhanced map coverage and global germplasm characterization. BMC Genomics 10:39

    Article  PubMed  Google Scholar 

  • Trevaskis B, Hemming MN, Dennis ES, Peacock WJ (2007) The molecular basis of vernalization-induced flowering in cereals. Trends Plant Sci 12:352–357

    Article  PubMed  CAS  Google Scholar 

  • Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78

    Article  PubMed  CAS  Google Scholar 

  • Wenzl P, Li H, Carling J, Zhou M, Raman H, Paul E, Hearnden P, Maier C, Xia L, Caig V, Ovesná J, Cakir M, Poulsen D, Wang J, Raman R, Smith KP, Muehlbauer GJ, Chalmers KJ, Kleinhofs A, Huttner E, Kilian A (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits. BMC Genomics 7:206

    Article  PubMed  Google Scholar 

  • Wittenberg AH, van der Lee T, Cayla C, Kilian A, Visser RG, Schouten HJ (2005) Validation of the high-throughput marker technology DArT using the model plant Arabidopsis thaliana. Mol Genet Genomics 274:30–39

    Article  PubMed  CAS  Google Scholar 

  • Zhang X-Y, Hu C-G, Yao J-L (2010) Tetraploidization of diploid Dioscorea results in activation of the antioxidant defense system and increased heat tolerance. J Plant Physiol 167:88–94

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

We are grateful to Marie Seifertová, MSc. for excellent technical assistance, and to the team at Diversity Arrays Technology Pty for DArT genotyping. This work has been supported by the Ministry of Agriculture of the Czech Republic (grant award NAZV QH71267) and by European Union (grant No. ED0007/01/01 Centre of the Region Haná for Biotechnological and Agricultural Research).

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Authors and Affiliations

  1. Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Sokolovská 6, 77200, Olomouc, Czech Republic

    Jan Bartoš, David Kopecký, Pavla Christelová, Štěpán Stočes & Jaroslav Doležel

  2. Department of Plant and Environmental Sciences, Norwegian University of Life Sciences, Ch. Magnus Falsens vei 1, 1432, Ås, Norway

    Simen Rød Sandve & Odd-Arne Rognli

  3. Agroscope Reckenholz-Tänikon Research Station ART, Reckenholzstrasse 191, 8046, Zurich, Switzerland

    Roland Kölliker

  4. Norwegian Institute for Agricultural and Environmental Research, Fureneset, 6997, Hellevik i Fjaler, Norway

    Liv Østrem

  5. Graminor AS, c/o Bioforsk Nord Bodø, Torggården, 8049, Bodø, Norway

    Arild Larsen

  6. Diversity Arrays Technology, 1 Wilf Crane Crescent, Yarralumla, ACT, 2600, Australia

    Andrzej Kilian

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  1. Jan Bartoš
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Correspondence to Jan Bartoš.

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Communicated by M. Xu.

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Bartoš, J., Sandve, S.R., Kölliker, R. et al. Genetic mapping of DArT markers in the Festuca–Lolium complex and their use in freezing tolerance association analysis. Theor Appl Genet 122, 1133–1147 (2011). https://doi.org/10.1007/s00122-010-1518-z

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