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Genotypic effects on the frequency of homoeologous and homologous recombination in Brassica napus × B. carinata hybrids

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Abstract

We investigated the influence of genotype on homoeologous and homologous recombination frequency in eight different Brassica napus (AACnCn) × B. carinata (BBCcCc) interspecific hybrids (genome composition CnCcAB). Meiotic recombination events were assessed through microsatellite marker analysis of 67 unreduced microspore-derived progeny. Thirty-four microsatellite markers amplified 83 A-, B-, Cn- and Cc-genome alleles at 64 loci, of which a subset of seven markers amplifying 26 alleles could be used to determine allele copy number. Hybrid genotypes varied significantly in loss of A- and B-genome alleles (P < 0.0001), which ranged from 6 to 22% between hybrid progeny sets. Allele copy number analysis revealed 19 A–C, 3 A–B and 10 B–C duplication/deletion events attributed to homoeologous recombination. Additionally, 55 deletions and 19 duplications without an accompanying dosage change in homoeologous alleles were detected. Hybrid progeny sets varied in observed frequencies of loss, gain and exchange of alleles across the A and B genomes as well as in the diploid C genome. Self-fertility in hybrid progeny decreased as the loss of B-genome loci (but not A-genome loci) increased. Hybrid genotypes with high levels of homologous and homoeologous exchange may be exploited for genetic introgressions between B. carinata and B. napus (canola), and those with low levels may be used to develop stable synthetic Brassica allopolyploids.

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Acknowledgments

This work was supported by the Australian Research Council Linkage Project LP0667805, with industry partners Council of Grain Grower Organisations Ltd and Norddeutsche Pflanzenzucht Hans-Georg Lembke KG. The majority of the molecular marker data were generated by Ms. Clare O’Lone. We thank Canola Breeders Western Australia Pty Ltd for provision of B. napus ‘Trilogy’, ‘Tristate’, ‘Trigold’ and ‘Surpass400-24DH’ for this study, and Dr. Anne-Marie Chèvre, INRA France, for her helpful suggestions on preparation of this manuscript.

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Authors and Affiliations

  1. School of Plant Biology and The UWA Institute of Agriculture, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia

    Annaliese S. Mason, Matthew N. Nelson, Marie-Claire Castello, Guijun Yan & Wallace A. Cowling

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  1. Annaliese S. Mason
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  2. Matthew N. Nelson
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  3. Marie-Claire Castello
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  4. Guijun Yan
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  5. Wallace A. Cowling
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Correspondence to Annaliese S. Mason.

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Communicated by P. Heslop-Harrison.

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Mason, A.S., Nelson, M.N., Castello, MC. et al. Genotypic effects on the frequency of homoeologous and homologous recombination in Brassica napus × B. carinata hybrids. Theor Appl Genet 122, 543–553 (2011). https://doi.org/10.1007/s00122-010-1468-5

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  • DOI: https://doi.org/10.1007/s00122-010-1468-5

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