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Molecular mapping reveals two independent loci conferring resistance to Albugo candida in the east European germplasm of oilseed mustard Brassica juncea

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Abstract

White rust caused by Albugo candida (Pers.) Kuntze is a major disease of the oilseed mustard Brassica juncea. Almost all the released varieties of B. juncea in India are highly susceptible to the disease. This causes major yield losses. Hence, there is an urgent need to identify genes for resistance to white rust and transfer these to the existing commercial varieties through marker-assisted breeding. While the germplasm belonging to the Indian gene pool is highly susceptible to the disease, the east European germplasm of B. juncea is highly resistant. In the present study, we have tagged two independent loci governing resistance to A. candida race 2V in two east European lines, Heera and Donskaja-IV. Two doubled haploid populations were used; the first population was derived from a cross between Varuna (susceptible Indian type) and Heera (partially resistant east European line) and the second from a cross between TM-4 (susceptible Indian type) and Donskaja-IV (fully resistant east European line). In both the resistant lines, a single major locus was identified to confer resistance to white rust. In Heera, the resistance locus AcB1-A4.1 was mapped to linkage group A4, while in Donskaja-IV, the resistant locus AcB1-A5.1 was mapped to linkage group A5. In both the cases, closely linked flanking markers were developed based on synteny between Arabidopsis and B. juncea. These flanking markers will assist introgression of resistance-conferring loci in the susceptible varieties.

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Acknowledgments

The work was supported by Mother Dairy Fruit and Vegetable Pvt. Ltd. (MDFVPL), a fully owned company of the National Dairy Development Board and the Department of Biotechnology, Government of India, under the Centre of Excellence on Genome Mapping and Molecular Breeding of Brassicas.

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Author notes

  1. Arvinder Kaur

    Present address: Rajmata Vijayaraje Scindia Krishi Vishwavidyalaya, Krishi Vigyan Kendra, Morena, India

Authors and Affiliations

  1. Centre for Genetic Manipulation of Crop Plants, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India

    Priya Panjabi-Massand, Arvinder Kaur, N. Arumugam, Yashpal Singh Sodhi, Arundhati Mukhopadhyay, Vibha Gupta, Akshay K. Pradhan & Deepak Pental

  2. Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India

    Satish Kumar Yadava, Pankaj Sharma, Amarendra Kumar, Akshay K. Pradhan & Deepak Pental

  3. Department of Plant Pathology, Agricultural Research Institute, Rajendra Agricultural University, Pusa, Lohiya Nagar, Patna, 800020, India

    Amarendra Kumar

  4. Department of Biotechnology, Pondicherry University, Pondicherry, 605014, India

    N. Arumugam

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  1. Priya Panjabi-Massand
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  2. Satish Kumar Yadava
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Corresponding author

Correspondence to Deepak Pental.

Additional information

Communicated by R. Visser.

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Fig 1S.

Host–pathogen interaction as visualized under light microscope after DAB staining. Hypersensitive response leading to the production of H2O2 was visualized as dark brown stained stomatal openings and adjacent cells in 3 days post-inoculated cotyledonary leaves of (a) Donskaja-IV and (b) Heera. No such hypersensitive response was observed in (c) Varuna. (PPT 15895 kb)

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Panjabi-Massand, P., Yadava, S.K., Sharma, P. et al. Molecular mapping reveals two independent loci conferring resistance to Albugo candida in the east European germplasm of oilseed mustard Brassica juncea . Theor Appl Genet 121, 137–145 (2010). https://doi.org/10.1007/s00122-010-1297-6

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