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Varietal and chromosome 2H locus-specific frost tolerance in reproductive tissues of barley (Hordeum vulgare L.) detected using a frost simulation chamber

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Abstract

Exposure of flowering cereal crops to frost can cause sterility and grain damage, resulting in significant losses. However, efforts to breed for improved low temperature tolerance in reproductive tissues (LTR tolerance) has been hampered by the variable nature of natural frost events and the confounding effects of heading time on frost-induced damage in these tissues. Here, we establish conditions for detection of LTR tolerance in barley under reproducible simulated frost conditions in a custom-built frost chamber. An ice nucleator spray was used to minimize potential effects arising from variation in naturally occurring extrinsic nucleation factors. Barley genotypes differing in their field tolerance could be distinguished. Additionally, an LTR tolerance quantitative trait locus (QTL) on the long arm of barley chromosome 2H could be detected in segregating families. In a recombinant family, the QTL was shown to be separable from the effects of the nearby flowering time locus Flt-2L. At a minimum temperature of −3.5°C for 2 h, detection of the LTR tolerance locus was dependent on the presence of the nucleator spray, suggesting that the tolerance relates to freezing rather than chilling, and that it is not the result of plant-encoded variation in ice-nucleating properties of the tiller surface.

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Acknowledgments

We gratefully acknowledge our funding bodies, the Australian Research Council, the Grains Research and Development Corporation, the South Australian Government and the University of Adelaide for financial support. We also thank Ursula Langridge and Robin Hosking for their assistance in the maintenance of growth rooms and greenhouses, and Joan Vickers from the University of Southern Queensland for helpful discussions and for provision of SNOMAX.

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Authors and Affiliations

  1. Australian Centre for Plant Functional Genomics (ACPFG), School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA, 5064, Australia

    Andrew Chen, Ute Baumann, Geoffrey B. Fincher & Nicholas C. Collins

  2. Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK, S7N 5A8, Canada

    Lawrence V. Gusta

  3. Department of Plant Science, Faculty of Agricultural and Food Sciences, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada

    Anita Brûlé-Babel

  4. Plant and Food Science, School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA, 5064, Australia

    Richard Leach

Authors
  1. Andrew Chen
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  2. Lawrence V. Gusta
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  3. Anita Brûlé-Babel
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  4. Richard Leach
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  5. Ute Baumann
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  6. Geoffrey B. Fincher
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  7. Nicholas C. Collins
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Corresponding author

Correspondence to Geoffrey B. Fincher.

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Communicated by F. Ordon.

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Chen, A., Gusta, L.V., Brûlé-Babel, A. et al. Varietal and chromosome 2H locus-specific frost tolerance in reproductive tissues of barley (Hordeum vulgare L.) detected using a frost simulation chamber. Theor Appl Genet 119, 685–694 (2009). https://doi.org/10.1007/s00122-009-1079-1

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  • DOI: https://doi.org/10.1007/s00122-009-1079-1

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