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A preliminary study on population genetic structure and phylogeography of the wild and cultivated Zizania latifolia (Poaceae) based on Adh1a sequences

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Abstract

Recent decades have witnessed growing interests in exploring the population genetics and phylogeography of crop plants and their wild relatives because of their important value as genetic resources. In this study, sequence variation of the nuclear Adh1a gene was used to investigate the genetic diversity and phylogeographic pattern of the wild and cultivated Zizania latifolia Turcz. Sequence data were obtained from 126 individuals representing 21 wild populations in China and 65 varieties of the cultivated Zizania latifolia. Low to medium level nucleotide diversity was found in the wild populations, with northeastern populations being the most variable. We detected significant population subdivision (F ST = 0.481) but no significant phylogeogaphical structure, suggesting limited gene flow and dispersal among populations. The current pattern of genetic variation in the wild populations might be explained by a fragmentation of ancient populations due to habitat destruction and degradation during recent decades. The heterogeneous levels and spatial apportionment of genetic diversity among wild populations also suggested a history of gradual colonization of Zizania latifolia populations from the northeast to the south of China. Interestingly, all 65 varieties of the cultivated Zizania latifolia possessed a single identical genotype, implying a single domestication associated with very few initial individuals.

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Acknowledgments

We thank Dan Yu and Sheng-lin Xia for help in field survey, and Feng Li and Xu-song Zheng for help in collection of cultivated varieties. We are also grateful to Liang Tang, Xin-hui Zou and Li-li Zang for providing primers, to Jin-xiu Wang for discussion, and to two anonymous reviewers for helpful comments and suggestions. This study was supported by the National Basic Research Program of China (2007CB815704) and National Natural Science Foundation of China (30121003) to Song Ge and a grant of the Excellent Overseas Young Scientists Program to Jun Wen.

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Authors and Affiliations

  1. State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China

    Xin-wei Xu & Song Ge

  2. Freshwater Ecological Field Station of Liangzi Lake, Wuhan University, Wuhan, 430072, China

    Xin-wei Xu

  3. National Garden of Aquatic Vegetable, Wuhan Institute of Vegetable Sciences, Hubei, 430065, China

    Wei-dong Ke

  4. Plant Protection and Microbe Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Xiao-ping Yu

  5. Department of Botany, National Museum of Natural History, Smithsonian Institute, MRC 166, Washington, DC, 20013-7012, USA

    Jun Wen

  6. Graduate School, Chinese Academy of Sciences, Beijing, 100039, China

    Song Ge

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  1. Xin-wei Xu
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  4. Jun Wen
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Correspondence to Song Ge.

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Communicated by J. Yu.

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Xu, Xw., Ke, Wd., Yu, Xp. et al. A preliminary study on population genetic structure and phylogeography of the wild and cultivated Zizania latifolia (Poaceae) based on Adh1a sequences. Theor Appl Genet 116, 835–843 (2008). https://doi.org/10.1007/s00122-008-0717-3

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