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Genetic diversity in accessions of lima bean (Phaseolus lunatus L.) determined from agro-morphological descriptors and SSR markers for use in breeding programs in Brazil

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Abstract

Lima bean (Phaseolus lunatus L.) have high genetic diversity, especially in Brazil, which has been maintained both on farms and in germplasm banks. Knowledge of the genetic diversity of germplasm in landraces is important for the development of breeding programs. We investigated the genetic diversity of lima bean landraces accessed in the Phaseolus Germplasm Bank of the Federal University of Piauí (PGB-UFPI, Brazil), using agro-morphological and microsatellite markers (SSRs). We analyzed 26 qualitative and 11 quantitative traits, following the Bioversity International descriptors. Furthermore, 15 SSRs were used to determine the genetic polymorphism and genetic differentiation among genotypes. The agro-morphological traits and SSR markers were reliable and effective for determining the genetic diversity of the accessions. Cluster analysis revealed a considerable phenotypic diversity among the accessions for some characters related to agronomic performance, such as the number of pods per plant, number of seeds per pod, and weight of 100 seeds. At the molecular level, ten loci showed polymorphism; the number of alleles per locus varied from two to seven, with mean polymorphism information content of 0.282 per locus. Loci GATS91 and PVat001 were highly informative and are indicated for further studies of lima bean. The accessions constitute an important gene pool for future breeding programs, especially accession UFPI-262. Accessions UFPI-262 and UFPI-252 can be used in crosses, as they are genetically distant and complementary in their characteristics.

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Funding

To the Coordination for the Improvement of Higher Education Personnel (CAPES) for the award of scholarships and financial support for the project Academic Cooperation to Enhance Post-graduation Formation in Genetics and Breeding (PROCAD UFPI/ESALQ), No. 23038.042963/2008-14. To the Luiz de Queiroz College of Agriculture (ESALQ), especially the Genetics Department, for making laboratory facilities available. To the National Council for Scientific and Technological Development (CNPq) for funding.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Genética e Melhoramento, Universidade Federal do Piauí, Campus Socopo, Teresina, PI, Brasil

    Carolline de Jesús Pires & João Paulo Gomes Viana

  2. Universidade Federal do Piauí, Campus Amílcar Ferreira Sobral, Floriano, PI, Brasil

    Marcones Ferreira Costa & José Ribamar Assunção-Filho

  3. Agência Paulista de Tecnologia dos Agronegócios, Pólo Centro-Sul, Piracicaba, SP, Brasil

    Maria Imaculada Zucchi

  4. Departamento de Genética, Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo, Piracicaba, SP, Brasil

    José Baldin Pinheiro

  5. Departamento de Engenharia de Pesca e Ciências Biológicas, Universidade do Estado de Santa Catarina, Laguna, SC, Brasil

    Miklos Maximiliano Bajay

  6. Departamento de Fitotecnia, Universidade Federal do Piauí, Teresina, PI, Brasil

    Regina Lucia Ferreira-Gomes & Ângela Celis de Almeida Lopes

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  1. Carolline de Jesús Pires
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  2. Marcones Ferreira Costa
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Contributions

Conceptualization: CJP, MIZ, RLFG, JBP, ACAL; formal analysis: CJP, JPGV, MMB, JRAF; investigation: CJP, JPGV, JRAF; writing, original draft: CJP, MFC; writing, revision, and editing: CJP, MFC, MMB, ACAL; supervision: MIZ, JBP, RLFG, ACAL; funding acquisition: JBP, RLFG, ACAL.

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Correspondence to Marcones Ferreira Costa.

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The geographical coordinates, accessions evaluated, and the readings of the SSRs are stored in our database and can be made available as requested.

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de Jesús Pires, C., Costa, M.F., Zucchi, M.I. et al. Genetic diversity in accessions of lima bean (Phaseolus lunatus L.) determined from agro-morphological descriptors and SSR markers for use in breeding programs in Brazil. Genet Resour Crop Evol 69, 973–986 (2022). https://doi.org/10.1007/s10722-021-01272-8

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