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Isolation and mapping of resistance gene analogs from the Avena strigosa genome

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Abstract

Degenerate primers based on conserved regions of the nucleotide binding site (NBS) domain (encoded by the largest group of cloned plant disease resistance genes) were used to isolate a set of 15 resistance gene analogs (RGA) from the diploid species Avena strigosa Schreb. These were grouped into seven classes on the basis of 60% or greater nucleic acid sequence identity. Representative clones were used for genetic mapping in diploid and hexaploid oats. Two RGAs were mapped at two loci of the linkage group AswBF belonging to the A. strigosa × A. wiestii Steud map, and ten RGAs were mapped at 15 loci in eight linkage groups belonging to the A. byzantina C. Koch cv. Kanota × A. sativa L. cv. Ogle map. A similar approach was used for targeting genes encoding receptor-like kinases. Three different sequences were obtained and mapped to two linkage groups of the hexaploid oat map. Associations were explored between already known disease resistance loci mapped in different populations and the RGAs. Molecular markers previously linked to crown rust and barley yellow dwarf resistance genes or quantitative trait loci were found in the Kanota × Ogle map linked to RGAs at a distance ranging from 0 cM to 20 cM. Homoeologous RGAs were found to be linked to loci either conferring resistance to different isolates of the same pathogen or to different pathogens. This suggests that these RGAs identify genome regions containing resistance gene clusters.

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Acknowledgements

We thank Dr. Mike Lee (Iowa State University, USA) for his kind contribution in mapping the diploid population. We also thank Drs. Ken Armstrong, Nick Tinker and Charlene Wight (Agriculture and Agri-Food, Canada) for providing us with the Kanota ×  Ogle seeds, for sharing unpublished data, and for helpful advice. Dr. Howard Rines (University of Minnesota, USA) is thanked for sharing preliminary data, and Eva Friero for technical assistance. This work was financed by the Spanish Ministry of Science and Technology (AG1999-0918).

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  1. Department of Cell Biology and Genetics, University of Alcalá, Campus Universitario, 28871 Alcalá de Henares, Madrid, Spain

    M. L. Irigoyen, Y. Loarce, A. Fominaya & E. Ferrer

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  1. M. L. Irigoyen
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  2. Y. Loarce
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  3. A. Fominaya
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Communicated by P. Langridge

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Irigoyen, M.L., Loarce, Y., Fominaya, A. et al. Isolation and mapping of resistance gene analogs from the Avena strigosa genome. Theor Appl Genet 109, 713–724 (2004). https://doi.org/10.1007/s00122-004-1679-8

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