Skip to main content
SpringerLink
Log in

Molecular mapping of k2 Mdh1-n y20, an unstable chromosomal region in soybean [Glycine max (L.) Merr.]

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

In the soybean genome, a chromosomal region covering three tightly linked genes, k2, Mdh1-n, and y20, was found very unstable. It was suspected that the instability of the k2 Mdh1-n y20 chromosomal region was caused by a non-autonomous transposable element residing adjacent to or in this region. In this study, we located and mapped this region with simple sequence repeat (SSR) markers on the soybean integrated map using five mapping populations. The k2 Mdh1-n y20 chromosomal region was located on molecular linkage group H. The integrated map from five mapping populations consisted of 13 loci in the order Satt541, Satt469, Sat_122, Satt279, Satt253, Satt314, Mdh1-n,y20, k2, Satt302, Satt142, Satt181, and Satt434. The k2 Mdh1-n y20 chromosomal region was very close to Satt314, Satt253, and Satt279. The genetic distance between the Mdh1-n gene and Satt314 was less than 1 cM. The results of the mapping study were consistent with the results from previous studies that the Mdh1-n mutation in T261 (k2 Mdh1-n) and the Mdh1-n y20 mutation in T317 (Mdh1-n y20) were caused by deletions. In addition, another putative deletion was found in the genome of T261 which covered three SSR markers (Satt314, Satt253, and Satt279).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Akkaya MS, Bhagwat AA, Cregan PB (1992) Length polymorphism of simple sequence repeat DNA in soybean. Genetics 132:1131–1139

    PubMed  CAS  Google Scholar 

  • Amberger LA, Shoemaker RC, Palmer RG (1992) Inheritance of two independent isozyme variants in soybean plants derived from tissue culture. Theor Appl Genet 84:600–607

    Article  Google Scholar 

  • Burzlaff JD, Palmer RG (1999) Soybean linkage studies: y18 and y20. Soybean Genet Newslett 26 (Online journal). http://www.soygenetics.org/articles/sgn1999-011.html (posted 23 April 1999)

  • Cardy BJ, Beversdorf WD (1984) A procedure for the starch gel electrophoretic detection of isozymes in soybean [Glycine max (L.) Merr.]. Dep Crop Sci Tech Bull 119–8401. Univ of Guelph, ON

  • Chen XF, Imsande J, Palmer RG (1999) Eight new mutants at the k2 Mdh1-n y20 chromosomal region in soybean. J Hered 90:339–403

    Google Scholar 

  • Chen XF, Palmer RG (1996) Inheritance and linkage with the k2 and Mdh1-n loci in soybean. J Hered 87:433–437

    CAS  Google Scholar 

  • Chen XF, Palmer RG (1998a) Recombination and linkage estimation between the k2 and Mdh1-n y20 loci in soybean. J Hered 89:488–494

    Article  CAS  Google Scholar 

  • Chen XF, Palmer RG (1998b) Instability at the k2 Mdh1-n y20 chromosomal region in soybean. Mol Gen Genet 260:309–318

    Article  CAS  Google Scholar 

  • Cregan PB, Jarvik T, Bush A, Shoemaker RC, Lark KG, Kahler A, Kaya N, VanToai T, Lohnes DG, Chung J, Specht JE (1999) An integrated genetic linkage map of the soybean genome. Crop Sci 39:1464–1490

    Article  CAS  Google Scholar 

  • Imsande J, Pittig J, Palmer RG, Wimmer C, Gietl C (2001) Independent spontaneous mitochondrial malate dehydrogenase null mutants in soybean are the result of deletions. J Hered 92:333–338

    Article  PubMed  CAS  Google Scholar 

  • Kato KK, Palmer RG (2003a) Genetic identification of a female partial-sterile mutant in soybean. Genome 46:128–134

    Article  PubMed  CAS  Google Scholar 

  • Kato KK, Palmer RG (2003b) Molecular mapping of male-sterile, female-sterile mutant gene (st8) in soybean. J Hered 94:425–428

    Article  PubMed  CAS  Google Scholar 

  • Kato KK, Palmer RG (2004a) Molecular mapping of four ovule lethal mutants in soybean. Theor Appl Genet 108:577–585

    Article  PubMed  CAS  Google Scholar 

  • Kato KK, Palmer RG (2004b) Duplicate chlorophyll-deficient loci in soybean. Genome 47:190–198

    Article  CAS  Google Scholar 

  • Keim P, Olson TC, Shoemaker RC (1988) A rapid protocol for isolating soybean DNA. Soybean Genet Newslett 15:150–152

    Google Scholar 

  • Kosambi DD (1944) The estimation of map distance from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  PubMed  CAS  Google Scholar 

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    Article  PubMed  CAS  Google Scholar 

  • Narvel JM, Jakkula LR, Phillips DV, Wang T, Lee SH, Boerma HR (2001) Molecular mapping of Rxp conditioning reaction to bacterial pustule in soybean. J Hered 92:267–270

    Article  PubMed  CAS  Google Scholar 

  • Palmer RG (1984) Pleiotropy or close linkage of two mutants in soybeans. J Hered 75:445–447

    Google Scholar 

  • Palmer RG, Burzlaff JD, Shoemaker RC (2000) Genetic analyses of two independent chlorophyll-deficient mutants identified among the progeny of a single chimeric foliage soybean plant. J Hered 91:297–303

    Article  PubMed  CAS  Google Scholar 

  • Palmer RG, Hedges BR, Benavente RS, Groose RW (1989) The w4 mutable line in soybean. Dev Genet 10:542–551

    Article  Google Scholar 

  • Palmer RG, Lim SM, Hedges BR (1992) Testing for linkage between the Rxp locus and nine isozyme loci in soybean. Crop Sci 32:681–683

    Article  CAS  Google Scholar 

  • Palmer RG, Pfeiffer TW, Buss GR, Kilen TC (2004) Qualitative genetics. In: Boerma HR, Specht JE (eds) Soybeans: improvement, production, and uses, 3rd edn. ASA, CSSA, SSSA, Madison, pp 137–214

    Google Scholar 

  • Pittig J, Guggenberger C, Palmer RG, Imsande J, Gietl C (1994) Molecular analysis of a deletion-prone chromosome segment in soybean [abstract 67]. Fourth international congress of plant molecular biology. International Society of Plant Molecular Biology, Amsterdam

  • Shoemaker RC, Olson TC (1993) Molecular linkage map of soybean (Glycine max L. Merr.). In: O’Brien SJ (ed) Genetic maps: locus maps of complex genomes. Cold Spring Harbor Laboratory Press, New York, pp 6.131–6.138

    Google Scholar 

  • Shoemaker RC, Polzin K, Labate J, Specht J, Brummer EC, Olson T, Young N, Concibido V, Wilcox J, Tamulonis JP, Kochert G, Boerma HR (1996) Genome duplication in soybean (Glycine subgenus soja). Genetics 144:329–339

    PubMed  CAS  Google Scholar 

  • Song QJ, Marek LF, Shoemaker RC, Lark KG, Concibido VC, Delannay X, Specht JE, Cregan PB (2004) A new integrated genetic linkage map of the soybean. Theor Appl Genet 109:122–128

    Article  PubMed  CAS  Google Scholar 

  • Stam P (1993) Construction of integrated genetic linkage maps by means of a new computer package: JoinMap. Plant J 3:739–744

    Article  CAS  Google Scholar 

  • Van Ooijen JW, Voorrips RE (2001) JoinMap® 3.0, software for the calculation of genetic linkage maps. Plant Research International, Wageningen

    Google Scholar 

  • Xu M, Palmer RG (2005) Genetic analysis and molecular mapping of a pale flower allele at the W4 locus in soybean. Genome 48:334–340

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

The authors would like to thank Dr. R. C. Shoemaker, USDA ARS and the Department of Agronomy, Iowa State University, Ames, IA, for providing the seeds of G. soja (PI 468.916) and A81-356022. The authors would also like to thank Dr. D. Grant, USDA ARS and the Department of Agronomy, Iowa State University, Ames, IA, for guidance in constructing maps using Mapmaker2.0.

Author information

Authors and Affiliations

  1. USDA ARS CICGR, Department of Agronomy, and Interdepartmental Genetics Graduate Program, Iowa State University, Ames, IA, 50011, USA

    Min Xu & Reid G. Palmer

Authors
  1. Min Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Reid G. Palmer
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Reid G. Palmer.

Additional information

Communicated by S. J. Knapp

This is a joint contribution of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa, Project No. 3769, and from the USDA, Agricultural Research Service, Corn Insects and Crop Genetics Research Unit, and supported by the Hatch Act and the State of Iowa. The mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by Iowa State University or the USDA, and the use of the name by Iowa State University or the USDA implies no approval of the product to the exclusion of others that may also be suitable.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xu, M., Palmer, R.G. Molecular mapping of k2 Mdh1-n y20, an unstable chromosomal region in soybean [Glycine max (L.) Merr.]. Theor Appl Genet 111, 1457–1465 (2005). https://doi.org/10.1007/s00122-005-0027-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-005-0027-y

Keywords

Search

Navigation