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Molecular mapping of four ovule lethal mutants in soybean

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Abstract

We report genetic mapping of four soybean ovule lethal mutants, PS-1, PS-2, PS-3, and PS-4, which had been identified as female partial-sterile mutants from a gene-tagging study. The four mutants had been classified into two mutation classes: (1) PS-1—sporophytic mutation affects sporophytically expressed genes; and (2) PS-2, PS-3, and PS-4 mutants—female gametophyte-specific mutations affect gametophytically expressed genes and are transmitted through the male, but not the female gametes. Molecular mapping demonstrated that these four mutant genes and previously reported female-partial sterile gene, Fsp1, are located independently on soybean molecular linkage groups (MLG-) using SSR markers. PS-1, designated as Fsp2 and Genetic Type Collection number T364, is located between SSR markers Satt170 and Satt363 on MLG-C2 and linked by 13.9 cM and 12.1 cM, respectively. PS-2, designated as Fsp3 and Genetic Type Collection number T365H, is located between SSR markers Satt538 and Satt429 on MLG-A2 and linked by 13.3 cM and 25.4 cM, respectively. PS-3, designated as Fsp4 and Genetic Type Collection number T366H, is located on the terminus of MLG-F and linked to Sat 152 by 13.1 cM. PS-4, designated as Fsp5 and Genetic Type Collection number T367H, is located between SSR markers Satt324 and Satt138 on MLG-G and linked by 19.6 cM and 7.5 cM, respectively. SSR markers adjacent to Fsp3, Fsp4, and Fsp5 were distorted from a 1:2:1 ratio and fit a 1:1 ratio. The segregation distortions of SSR markers adjacent to Fsp3, Fsp4, and Fsp5 are in support of male, but not female transmission of the Fsp3, Fsp4, and Fsp5 gametes.

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Acknowledgements

The authors wish to thank Dr. P.B. Cregan, USDA-ARS Soybean and Alfalfa Research Laboratory, Beltsville, Md., USA and Monsanto Company for giving us their unpublished SSR primer sequence information.

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Authors and Affiliations

  1. Department of Crop Science, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, 080-8555, Hokkaido, Japan

    K. K. Kato

  2. USDA ARS CICGR and Departments of Agronomy and of Zoology/Genetics, Iowa State University, Ames, IA 50011-1010, USA

    R. G. Palmer

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  1. K. K. Kato
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  2. R. G. Palmer
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Correspondence to R. G. Palmer.

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This is a joint contribution of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa, Project No. 3769 and from the USDA, Agricultural Research Service, Corn Insects and Crop Genetics Research Unit, and supported by Hatch Act and State of Iowa. The mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by Iowa State University or the USDA, and the use of the name by Iowa State University or the USDA implies no approval of the product to the exclusion of others that may also be suitable.

Communicated by J. Dvorak

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Kato, K.K., Palmer, R.G. Molecular mapping of four ovule lethal mutants in soybean. Theor Appl Genet 108, 577–585 (2004). https://doi.org/10.1007/s00122-003-1482-y

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