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Microsatellite markers associated with two Aegilops tauschii-derived greenbug resistance loci in wheat

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Abstract

A new source of greenbug (Schizaphis graminum Rondani) resistance derived from Aegilops tauschii (Coss.) Schmal was identified in W7984, a synthetic hexaploid wheat line and one parent of the International Triticeae Mapping Initiative (ITMI) mapping population. Segregation analysis of responses to greenbug feeding in a set of recombinant inbred lines (RILs) identified a single, dominant gene governing the greenbug resistance in W7984, which was placed in chromosome arm 7DL by linkage analysis with molecular markers in the ITMI population. Allelism tests based on the segregation of responses to greenbug feeding in F2 and testcross plants revealed that the greenbug resistance in W7984 and Largo, another synthetic line carrying the greenbug resistance gene Gb3, was controlled by different but linked loci. Using the ITMI reference map and a target mapping strategy, we have constructed a microsatellite map of Gb3 in a mapping population of 130 F7 RILs from Largo × TAM 107 and identified one marker (Xwmc634) co-segregating with Gb3 and four markers (Xbarc76, Xgwm037, Xgwm428 and Xwmc824) closely linked with Gb3. Deletion mapping of selected microsatellite markers flanking the Gb3 locus placed this resistance gene into the distal 18% region of 7DL. Comparative mapping in the ITMI and Largo × TAM 107 populations using the same set of microsatellite markers provided further evidence that greenbug resistance in W7984 and Largo is conditioned by two different loci. We suggest that the greenbug resistance gene in W7984 be designated Gb7. The microsatellite map of Gb3 constructed from this study should be a valuable tool for marker-assisted selection of Gb3-conferred greenbug resistance in wheat breeding.

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Acknowledgements

We are indebted to Peihua Yan and Gary Peterson for technical help. The authors thank Drs. David R. Porter (USDA-ARS, Stillwater, Okla., USA), Calvin O. Qualset (University of California, Davis, Calif., USA) and Bikram S. Gill (Kansas State University, Manhattan, Kan., USA) for providing greenbugs or plant materials for this study. We also thank Dr. Daryl Somers (Cereal Research Center, Winnipeg, Canada) for providing the primer sequences of four WMC microsatellite markers. This research was supported by a grant from the NRICGP program of the United States Department of Agriculture (2002-35301-12044) to Y.W.

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Authors and Affiliations

  1. Texas A&M University Agricultural Research and Extension Center, 6500 Amarillo Blvd. W., Amarillo, TX, 79106, USA

    Y. Weng, R. N. Devkota & J. C. Rudd

  2. Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506, USA

    W. Li

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  1. Y. Weng
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  2. W. Li
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  3. R. N. Devkota
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  4. J. C. Rudd
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Correspondence to Y. Weng.

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Communicated by F. Salamini

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Weng, Y., Li, W., Devkota, R.N. et al. Microsatellite markers associated with two Aegilops tauschii-derived greenbug resistance loci in wheat. Theor Appl Genet 110, 462–469 (2005). https://doi.org/10.1007/s00122-004-1853-z

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  • DOI: https://doi.org/10.1007/s00122-004-1853-z

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