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Exploiting selective genotyping to study genetic diversity of resistance to Fusarium head blight in barley

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Abstract

Numerous barley cultivars from around the world have been identified as potential sources of Fusarium head blight (FHB) resistance genes. All of these cultivars exhibit partial resistance, and several mapping studies have shown that resistance to FHB is controlled by multiple genes. Successful development of barley cultivars with high levels of FHB resistance will require combining genes from multiple sources. We characterized five potential new sources of FHB resistance (‘AC Oxbow’, ‘Atahualpa’, ‘HOR211’, ‘PFC88209’, and ‘Zhedar#1’) to determine if they contain new FHB resistance genes. Cluster analysis, using a set of 80 SSR markers distributed throughout the genome, showed that most of the new sources of resistance were not similar to three cultivars that have been used in previous FHB mapping studies (‘Chevron’, ‘Frederickson’, and ‘Gobernadora’), with ‘Atahualpa’ and ‘HOR211’ being the most dissimilar. By selective genotyping, we determined whether markers linked to six known FHB resistance quantitative trait loci (QTLs), discovered in other genotypes, explained variation for resistance in advanced breeding populations created from the new sources of resistance. Markers linked to four of the six known QTLs were associated with FHB severity in at least one of the populations. However, none of the six QTL regions were associated with variation for FHB severity in populations derived from crosses that utilized sources of resistance HOR211 or PFC88209. Selective genotyping is an efficient method for breeders to utilize current QTL information about disease resistance to search for new resistance genes.

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Acknowledgements

We thank R. Bernardo, R. Dill-Macky, and E. Schiefelbein for valuable assistance and members of the Minnesota Small Grains Pathology and Barley Breeding Programs for help in disease screening. We also thank D. Garvin for reviewing an earlier draft of this manuscript. This research was supported, in part, through grants provided by the US Wheat and Barley Scab Initiative, the American Malting Barley Association, and the Minnesota Small Grains Initiative.

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  1. Department of Agronomy and Plant Genetics, University of Minnesota, Rm 411 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, MN, 55108, USA

    W. J. Wingbermuehle, C. Gustus & K. P. Smith

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  1. W. J. Wingbermuehle
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  2. C. Gustus
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  3. K. P. Smith
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Correspondence to K. P. Smith.

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Communicated by D. Hoisington

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Wingbermuehle, W.J., Gustus, C. & Smith, K.P. Exploiting selective genotyping to study genetic diversity of resistance to Fusarium head blight in barley. Theor Appl Genet 109, 1160–1168 (2004). https://doi.org/10.1007/s00122-004-1733-6

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