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Genetic analysis of male fertility restoration in wild cytoplasmic male sterility G of beet

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Abstract

Cytoplasmic male sterility (CMS) has been used in the breeding of sugar beet for decades but is also more generally an important feature of the reproductive system in its wild relative, Beta vulgaris ssp. maritima. Among the several CMSs found in wild populations, the G CMS is a mitochondrial variant of the respiratory chain. The segregants derived from a cross between a restored plant and a female (male-sterile) plant on G cytoplasm exhibited three sexual phenotypic classes: female, hermaphrodite and intermediate. The pattern of segregation suggests a genetic inheritance with two loci in epistatic interaction. Nevertheless, it was possible to apply a bulk segregant analysis approach to search for AFLP and microsatellite markers linked to the restorer locus (RfG 1 ) which controls the capacity to produce pollen [female versus non female (i.e. intermediates and hermaphrodites)] in the segregating population. A linkage group was constructed with four AFLP markers and nine microsatellites, and a total size of 40 cM (Kosambi). The closest marker, a microsatellite, was totally linked to RfG1, which was also flanked by two AFLP markers delimiting a 5 cM window. This linkage group was identified as being chromosome VIII where neither of the restorer loci of the Owen CMS are located.

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Acknowledgements

We thank Anne-Sophie Wepierre and Marianne De Paepe for preliminary flower phenotyping, Robert Dron for his technical expertise in plant care, Hans Koelewijn and Jean-François Arnaud for valuable comments on the manuscript. This work was supported by grants from the Région Nord-Pas de Calais and the European Community (European Regional Development Fund).

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Authors and Affiliations

  1. Laboratoire de Génétique et Evolution des Populations Végétales, UMR-CNRS 8016, FR CNRS 1818, Université de Lille I, 59655 , Villeneuve d’Ascq , Cedex, France

    Pascal Touzet, Nathalie Hueber & Joël Cuguen

  2. Advanta, SES Europe, Industriepark, Soldatenplein Z2 nr 15, 3300, Tienen , Belgium

    Alexandra Bürkholz & Stephen Barnes

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  1. Pascal Touzet
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  2. Nathalie Hueber
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  3. Alexandra Bürkholz
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  4. Stephen Barnes
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  5. Joël Cuguen
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Correspondence to Pascal Touzet.

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Communicated by H.C. Becker

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Touzet, P., Hueber, N., Bürkholz, A. et al. Genetic analysis of male fertility restoration in wild cytoplasmic male sterility G of beet. Theor Appl Genet 109, 240–247 (2004). https://doi.org/10.1007/s00122-004-1627-7

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  • DOI: https://doi.org/10.1007/s00122-004-1627-7

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