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A genetic linkage map of the diplosporous chromosomal region in Taraxacum officinale (common dandelion; Asteraceae)

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Abstract

In this study, we mapped the diplosporous chromosomal region in Taraxacum officinale, by using amplified fragment length polymorphism technology (AFLP) in 73 plants from a segregating population. Taraxacum serves as a model system to investigate the genetics, ecology, and evolution of apomixis. The genus includes sexual diploid as well as apomictic polyploid, mostly triploid, plants. Apomictic Taraxacum is diplosporous, parthenogenetic, and has autonomous endosperm formation. Previous studies have indicated that these three apomixis elements are controlled by more than one locus in Taraxacum and that diplospory inherits as a dominant, monogenic trait (Ddd; DIP). A bulked segregant analysis provided 34 AFLP markers that were linked to DIP and were, together with two microsatellite markers, used for mapping the trait. The map length was 18.6 cM and markers were found on both sides of DIP, corresponding to 5.9 and 12.7 cM, respectively. None of the markers completely co-segregated with DIP. Eight markers were selected for PCR-based marker development, of which two were successfully converted. In contrast to all other mapping studies of apomeiosis to date, our results showed no evidence for suppression of recombination around the DIP locus in Taraxacum. No obvious evidence for sequence divergence between the DIP and non-DIP homologous loci was found, and no hemizygosity at the DIP locus was detected. These results may indicate that apomixis is relatively recent in Taraxacum.

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Acknowledgements

This research is part of the European Union funded project: “Natural apomixis as a novel tool in plant breeding (ApoTool)”, contract number QLG2-2000-00603 of the Quality of Life and Management of Living Resources section. This paper corresponds to publication number 3225 of the Netherlands Institute of Ecology (NIOO-KNAW).

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Authors and Affiliations

  1. Centre for Terrestrial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Boterhoeksestraat 48, 6666 GA, Heteren, The Netherlands

    K. Vijverberg & P. J. Van Dijk

  2. Department of Plant Science, Laboratory of Plant Breeding, Wageningen University and Research Centre, Binnenhaven 5, 6700 AJ, Wageningen, The Netherlands

    R. G. M. Van der Hulst & P. Lindhout

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  1. K. Vijverberg
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  2. R. G. M. Van der Hulst
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  3. P. Lindhout
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  4. P. J. Van Dijk
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Correspondence to K. Vijverberg.

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Communicated by C. Möllers

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Vijverberg, K., Van der Hulst, R.G.M., Lindhout, P. et al. A genetic linkage map of the diplosporous chromosomal region in Taraxacum officinale (common dandelion; Asteraceae). Theor Appl Genet 108, 725–732 (2004). https://doi.org/10.1007/s00122-003-1474-y

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