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Narrowing down the region of the Vf locus for scab resistance in apple using AFLP-derived SCARs

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Abstract

A narrow-down strategy to restrict the Vf region, which controls resistance to the fungal disease apple scab in apple, to a genetic distance of 0.4 cM is presented. Using 11 AFLP-derived SCARs and three RAPD-derived SCARs, all linked to the Vf gene, we subjected 1,412 scab-resistant individuals from 16 mapping populations to genotype analysis. Eleven recombinant individuals were identified within a genetic distance of 0.9 cM around the Vf gene. Using these 11 recombinants, we achieved fine-resolution of several AFLP-derived SCAR markers surrounding the Vf gene, resulting in the following genetic linkage map: ACS-6 and ACS are located left of the Vf gene at genetic distances of 0.2 cM and 0.1 cM, respectively; ACS-7 and ACS-9 are inseparable from the Vf gene; ACS-8, ACS-10, and ACS-4 are located to the right of the Vf gene at genetic distances of 0.1 cM, 0.4 cM, and 0.5 cM, respectively; the remaining five SCARs—ACS-11, ACS-5, ACS-2, ACS-1, and AL07—are inseparable and are located right of the Vf gene at a genetic distance of 0.7 cM. By integrating this linkage data with our previous physical map, we generated a revised map of the narrowed-down region of Vf.

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Acknowledgements

This project was supported by funds from the Illinois Council for Food and Agricultural Research (C-FAR) External program and the Office of Research project no. 65-325.

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  1. M. Xu

    Present address: College of Bioscience and Biotechnology, Yangzhou University, Jiangsu, Yangzhou, P.R. China

Authors and Affiliations

  1. Department of Natural Resources and Environmental Sciences, University of Illinois, 310 Madigan Building, 1201 West Gregory Drive, Urbana, IL, 61801, USA

    E. Huaracha, M. Xu & S. S. Korban

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  1. E. Huaracha
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  2. M. Xu
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  3. S. S. Korban
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Correspondence to S. S. Korban.

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Communicated by P. Langridge

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Huaracha, E., Xu, M. & Korban, S.S. Narrowing down the region of the Vf locus for scab resistance in apple using AFLP-derived SCARs. Theor Appl Genet 108, 274–279 (2004). https://doi.org/10.1007/s00122-003-1435-5

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