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Genetic diversity in recent elite faba bean lines using AFLP markers

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Abstract.

Amplified fragment length polymorphism (AFLP) markers were used to study the genetic diversity among a large set (n = 79) of inbred lines of recent elite faba bean (Vicia faba L.) cultivars with Asian, European (Northern and Southern) and North African origin. The inbred lines were analyzed using eight selected AFLP primer combinations that produced 477 polymorphic fragments. Errors when scoring repeated lanes of one pre-amplification reaction on one gel were negligible, whereas errors when scoring lanes of two individuals of the same inbred line run on different gels were markedly higher. Scoring across gels should be backed by replicates and several appropriate check entries. Based on clustering with Jaccard's similarity coefficient and Principal Coordinate Analysis, only the Asian lines were distinct as a group, the other lines showed no marked further grouping. Nevertheless, several known pedigree relationships were verified. A priori grouping of inbred lines (geographic origin and seed size) and AFLP data corroborate available information on the history of spread and cultivation of faba bean in the studied regions. Based on the diversity observed, studies especially concerning the relationship between genetic similarity based on AFLP markers and hybrid performance within the European elite germplasm have been launched.

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Acknowledgements.

Thanks are due to all who provided us with the seed material used in this study, especially to Abbate (Sicily), Abdelmula (Sudan), Duc (France), Ebmeyer (Germany), Fukuta (Japan), Lang li-juan (China), Larissa (Greece), Pope (UK), Sass (Germany), Stringi (Sicily) and Winkler (Austria), for their expertise and valuable information concerning the provided material. The financial support from the Gemeinschaft zur Förderung der privaten deutschen Pflanzenzüchtung e. V. (GFP) is gratefully acknowledged.

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Authors and Affiliations

  1. Institute of Agronomy and Plant Breeding, Georg-August-University, 37075 Göttingen, Germany

    Mahmoud Zeid & Wolfgang Link

  2. State Plant Breeding Institute, University of Hohenheim, 70593 Stuttgart, Germany

    Chris-Carolin Schön

Authors
  1. Mahmoud Zeid
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  2. Chris-Carolin Schön
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Correspondence to Wolfgang Link.

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Communicated by H.F. Linskens

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Zeid, M., Schön, CC. & Link, W. Genetic diversity in recent elite faba bean lines using AFLP markers. Theor Appl Genet 107, 1304–1314 (2003). https://doi.org/10.1007/s00122-003-1350-9

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  • DOI: https://doi.org/10.1007/s00122-003-1350-9

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