Abstract
We have tried an insect-pollination self-incompatibility (SI) test to strictly evaluate the level of SI as a model for the actual F1 seed production field using radish as experimental material. Twelve inbred lines, homozygous for the S-alleles, were used in the artificial self-pollination and the insect-pollination SI test. There was a positive correlation (r = 0.606) between the results by these two methods. Some lines showed a low level of SI in the insect-pollination test despite showing a high level of SI in the artificial self-pollination test. On the other hand, no lines showing a low level of SI in artificial self-pollination had a high level of SI in insect-pollination. These results show that the insect-pollination SI test can be considered to be a more reliable and stricter method than the artificial self-pollination test with respect to an evaluation of SI levels. We have raised and analyzed an F2 population and F3 lines derived from an F1 cross between a line showing a high level of SI (R00-04) and one showing a low level. The rate of self-seed settings of the F2 population showed a binomial distribution. There were 39 high-level SI plants to 15 low-level SI plants. This result and F3 progeny tests suggested that the high level of SI which R00-04 showed is controlled by a dominant gene.
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Acknowledgements
We thank Dr. Y. Fujita, an executive director of Tohoku Seed Company, for his support and Dr. S. Matsuura of Tohoku Seed Company for his generous advise during this study.
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Communicated by H.F. Linskens
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Horisaki, A., Tanaka, N. & Niikura, S. The effectiveness of insect-pollination test to evaluate the level of self-incompatibility and their genetic analysis in radish (Raphanus sativus L.). Theor Appl Genet 107, 1009–1013 (2003). https://doi.org/10.1007/s00122-003-1256-6
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DOI: https://doi.org/10.1007/s00122-003-1256-6