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AFLP genetic polymorphism in wild barley (Hordeum spontaneum) populations in Israel

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An Erratum to this article was published on 23 April 2003

Abstract.

The genetic diversity produced by the amplified fragment length polymorphism (AFLP) method was studied in 94 genotypes of wild barley, Hordeum spontaneum (C. Koch) Thell., originating from ten ecologically and geographically different locations in Israel. Eight primer pairs produced 204 discernible loci of which 189 (93%) were polymorphic. Each genotype had a unique banding profile and the genetic similarity coefficient varied between 0.74 and 0.98. The phenogram generated from these similarities by the UPGMA method did not group genotypes strictly according to their geographical origin, which pattern was also seen in the principal coordinate (PCO) plot. Genetic diversity was larger within (69%) than among (31%) populations. Associations between ecogeographical variables and the mean gene diversity were found at one primer pair. The results are discussed and compared with data obtained by the simple sequence repeat (SSR) method.

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Acknowledgements.

We gratefully acknowledge Ms. Teija Tenhola for growing leaf material and Ms. Leena Lohermaa and Ms. Sirpa Moisander for the DNA extractions. T. Turpeinen acknowledges the Finnish Cultural Foundation for financial support. E. Nevo thanks the Ancell-Teicher Research Foundation for Genetics and Molecular Evolution.

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Authors and Affiliations

  1. MTT Agrifood Research Finland, Plant Production Research, Crops and Biotechnology, FIN-31600 Jokioinen, Finland

    T. Turpeinen

  2. Laboratory of Plant Breeding, Department of Plant Sciences, Wageningen University, P.O.Box 386, 6700 AJ Wageningen, Netherlands

    T. Vanhala

  3. Institute of Evolution, University of Haifa, Mt. Carmel, Haifa 31905, Israel

    E. Nevo

  4. Boreal Plant Breeding Ltd, FIN-31600 Jokioinen, Finland

    E. Nissilä

Authors
  1. T. Turpeinen
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  2. T. Vanhala
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  3. E. Nevo
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  4. E. Nissilä
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Correspondence to T. Turpeinen.

Additional information

Communicated by H.F. Linskens

T. Turpeinen and T. Vanhala contributed equally to this work

An erratum to this article is available at http://dx.doi.org/10.1007/s00122-003-1286-0.

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Turpeinen, T., Vanhala, T., Nevo, E. et al. AFLP genetic polymorphism in wild barley (Hordeum spontaneum) populations in Israel. Theor Appl Genet 106, 1333–1339 (2003). https://doi.org/10.1007/s00122-002-1151-6

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  • DOI: https://doi.org/10.1007/s00122-002-1151-6

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